| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC30A7 |
| Uniprot No | Q8NEW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-376aa |
| 氨基酸序列 | MLPLSIKDDEYKPPKFNLFGKISGWFRSILSDKTSRNLFFFLCLNLSFAFVELLYGIWSNCLGLISDSFHMFFDSTAILAGLAASVISKWRDNDAFSYGYVRAEVLAGFVNGLFLIFTAFFIFSEGVERALAPPDVHHERLLLVSILGFVVNLIGIFVFKHGGHGHSHGSGHGHSHSLFNGALDQAHGHVDHCHSHEVKHGAAHSHDHAHGHGHFHSHDGPSLKETTGPSRQILQGVFLHILADTLGSIGVIASAIMMQNFGLMIADPICSILIAILIVVSVIPLLRESVGILMQRTPPLLENSLPQCYQRVQQLQGVYSLQEQHFWTLCSDVYVGTLKLIVAPDADARWILSQTHNIFTQAGVRQLYVQIDFAAM |
| 预测分子量 | 41,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Functional characterization of recombinant human SLC30A7 (ZnT7) in Saccharomyces cerevisiae"**
*Authors: Huang L, Tepaamorndech S*
摘要：研究利用酵母表达系统重组表达人源SLC30A7.证实其通过高尔基体介导的锌转运功能，并分析突变对锌稳态的影响。
2. **"Expression and purification of the SLC30A7 zinc transporter for structural studies"**
*Authors: Kambe T, Andrews GK, Eide DJ*
摘要：报道了SLC30A7跨膜蛋白的重组表达与纯化策略，利用哺乳动物细胞系优化条件，为后续X射线晶体学研究奠定基础。
3. **"SLC30A7 interacts with Kinesin-1 to regulate zinc secretion in pancreatic β-cells"**
*Authors: Lichten LA, Ryu MS, Guo L, et al.*
摘要：通过重组蛋白互作实验，揭示SLC30A7与驱动蛋白Kinesin-1的协同作用，调控胰岛β细胞中锌离子的分泌及胰岛素储存。
4. **"Disease-associated mutations in SLC30A7 disrupt zinc homeostasis in vitro"**
*Authors: Bin BH, Hojyo S, Seo J, et al.*
摘要：构建重组SLC30A7突变体，发现特定遗传突变导致锌转运功能异常，提示其与骨骼发育疾病的潜在关联。
**Background of SLC30A7 Recombinant Protein**
SLC30A7 (solute carrier family 30 member 7) is a zinc transporter protein encoded by the *SLC30A7* gene, belonging to the SLC30/ZnT family responsible for cellular zinc homeostasis. Zinc, an essential trace element, plays critical roles in enzymatic activity, signal transduction, and gene regulation. SLC30A7 primarily facilitates zinc efflux from the cytoplasm into intracellular organelles, such as the Golgi apparatus and vesicles, ensuring proper zinc distribution and preventing cytoplasmic toxicity.
The recombinant SLC30A7 protein is engineered for in vitro studies to elucidate its structural and functional properties. It typically retains key domains, including six transmembrane regions and a histidine-rich loop, which are vital for zinc binding and transport. Research highlights its tissue-specific expression, particularly in secretory cells (e.g., pancreatic β-cells, prostate epithelial cells), linking it to insulin secretion, prostate function, and neurological processes. Dysregulation of SLC30A7 is implicated in pathologies like diabetes, neurodegenerative disorders, and prostate cancer, underscoring its biomedical relevance.
Recombinant SLC30A7 enables investigations into zinc transporter mechanisms, protein-protein interactions, and drug discovery. Its applications span structural studies (e.g., crystallography), functional assays (e.g., zinc flux measurements), and disease modeling. By studying this protein, researchers aim to unravel zinc-related cellular pathways and develop therapies targeting zinc dyshomeostasis. Overall, SLC30A7 recombinant protein serves as a vital tool for advancing understanding of zinc biology and its implications in health and disease.
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