| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC30A6 |
| Uniprot No | Q6NXT4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461aa |
| 氨基酸序列 | MGTIHLFRKPQRSFFGKLLREFRLVAADRRSWKILLFGVINLICTGFLLMWCSSTNSIALTAYTYLTIFDLFSLMTCLISYWVTLRKPSPVYSFGFERLEVLAVFASTVLAQLGALFILKESAERFLEQPEIHTGRLLVGTFVALCFNLFTMLSIRNKPFAYVSEAASTSWLQEHVADLSRSLCGIIPGLSSIFLPRMNPFVLIDLAGAFALCITYMLIEINNYFAVDTASAIAIALMTFGTMYPMSVYSGKVLLQTTPPHVIGQLDKLIREVSTLDGVLEVRNEHFWTLGFGSLAGSVHVRIRRDANEQMVLAHVTNRLYTLVSTLTVQIFKDDWIRPALLSGPVAANVLNFSDHHVIPMPLLKGTDDLNPVTSTPAKPSSPPPEFSFNTPGKNVNPVILLNTQTRPYGFGLNHGHTPYSSMLNQGLGVPGIGATQGLRTGFTNIPSRYGTNNRIGQPRP |
| 预测分子量 | 51,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC30A6重组蛋白的模拟参考文献(非真实文献，仅供示例)：
1. **文献名称**：Structural and functional analysis of recombinant human SLC30A6 zinc transporter
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化SLC30A6重组蛋白，利用X射线晶体学解析其三维结构，揭示其锌离子结合位点及跨膜转运机制。
2. **文献名称**：SLC30A6 recombinant protein expression in mammalian cells and its role in insulin secretion
**作者**：Li X, et al.
**摘要**：在HEK293细胞中重组表达SLC30A6蛋白，证实其通过调节胞内锌离子浓度影响胰岛素囊泡释放，为2型糖尿病机制研究提供依据。
3. **文献名称**：Characterization of SLC30A6 knockout and recombinant rescue in zebrafish models
**作者**：Wang Y, et al.
**摘要**：利用斑马鱼模型研究SLC30A6功能缺失表型，通过注射重组SLC30A6蛋白恢复锌稳态，证明其在胚胎发育中的必要性。
4. **文献名称**：Development of a high-yield SLC30A6 recombinant protein production system using Pichia pastoris
**作者**：Garcia R, et al.
**摘要**：优化毕赤酵母表达系统，实现SLC30A6重组蛋白的高效分泌表达，并验证其体外锌转运活性，为药物筛选提供工具。
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索。
The solute carrier family 30 member 6 (SLC30A6), also known as zinc transporter 6 (ZnT-6), is a transmembrane protein encoded by the SLC30A6 gene in humans. As part of the SLC30/ZNT family, it plays a critical role in zinc homeostasis by mediating zinc efflux from the cytoplasm into intracellular organelles or extracellular spaces. Zinc, an essential trace element, is vital for enzymatic activity, signal transduction, and cellular metabolism. SLC30A6 is predominantly expressed in the brain, pancreas, and gastrointestinal tissues, where it regulates zinc distribution to support physiological processes such as insulin secretion, neuronal function, and immune responses.
Recombinant SLC30A6 protein refers to the engineered form of this transporter produced in vitro using expression systems like bacterial, yeast, or mammalian cell cultures. Its production enables detailed biochemical and structural studies to elucidate zinc transport mechanisms, substrate specificity, and interactions with other proteins. Researchers utilize recombinant SLC30A6 to investigate its role in diseases linked to zinc dysregulation, including type 2 diabetes, neurodegenerative disorders (e.g., Alzheimer’s), and cancers. Structural analysis of the recombinant protein, often via X-ray crystallography or cryo-EM, provides insights into its transmembrane domain organization and zinc-binding sites.
Current studies focus on SLC30A6’s potential as a therapeutic target, particularly in modulating zinc levels to treat metabolic or neurological conditions. Challenges include optimizing recombinant protein stability and mimicking native post-translational modifications for functional accuracy. Ongoing research aims to clarify its regulatory pathways and tissue-specific roles, advancing both basic science and clinical applications.
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