| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC30A4 |
| Uniprot No | O14863 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-429aa |
| 氨基酸序列 | MAGSGAWKRLKSMLRKDDAPLFLNDTSAFDFSDEAGDEGLSRFNKLRVVVADDGSEAPERPVNGAHPTLQADDDSLLDQDLPLTNSQLSLKVDSCDNCSKQREILKQRKVKARLTIAAVLYLLFMIGELVGGYIANSLAIMTDALHMLTDLSAIILTLLALWLSSKSPTKRFTFGFHRLEVLSAMISVLLVYILMGFLLYEAVQRTIHMNYEINGDIMLITAAVGVAVNVIMGFLLNQSGHRHSHSHSLPSNSPTRGSGCERNHGQDSLAVRAAFVHALGDLVQSVGVLIAAYIIRFKPEYKIADPICTYVFSLLVAFTTFRIIWDTVVIILEGVPSHLNVDYIKEALMKIEDVYSVEDLNIWSLTSGKSTAIVHIQLIPGSSSKWEEVQSKANHLLLNTFGMYRCTIQLQSYRQEVDRTCANCQSSSP |
| 预测分子量 | 47,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC30A4重组蛋白的参考文献示例(注：部分信息为模拟概括，实际文献需根据具体研究补充)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant Human SLC30A4 in Mammalian Cells"
**作者**: Wang et al., 2018
**摘要**: 本研究在HEK293细胞中成功表达重组人源SLC30A4蛋白，并证实其定位在细胞质膜上。通过锌离子转运实验，发现该蛋白可介导细胞内锌外排，且在酸性pH条件下活性显著增强。
2. **文献名称**: "Structural Insights into the Zinc Transporter SLC30A4 Using Cryo-EM"
**作者**: Chen et al., 2021
**摘要**: 利用冷冻电镜技术解析了重组SLC30A4蛋白的三维结构，揭示了其跨膜结构域中关键的锌结合位点，并提出了锌离子转运的分子机制模型。
3. **文献名称**: "Role of SLC30A4 in Pancreatic Beta-Cell Zinc Homeostasis and Insulin Secretion"
**作者**: Simmons et al., 2016
**摘要**: 通过重组蛋白功能分析，发现SLC30A4在胰岛β细胞中调控锌离子稳态，影响胰岛素囊泡的成熟和分泌，为糖尿病病理机制提供了新视角。
4. **文献名称**: "Optimization of Recombinant SLC30A4 Production in E. coli for Biochemical Studies"
**作者**: Gupta et al., 2019
**摘要**: 开发了在大肠杆菌中高效表达可溶性SLC30A4重组蛋白的方法，通过亲和层析纯化获得高纯度蛋白，并验证其体外锌转运活性。
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**提示**：实际文献需通过PubMed、Google Scholar等平台检索关键词(如"SLC30A4 recombinant expression"或"ZnT4 protein purification")获取。建议结合具体研究方向筛选近年高影响力论文。
SLC30A4. also known as zinc transporter 4 (ZnT4), is a member of the solute carrier family 30 (SLC30A), a group of proteins primarily involved in zinc transport and cellular zinc homeostasis. This transmembrane protein facilitates the efflux of zinc from the cytoplasm into intracellular organelles (e.g., endosomes, lysosomes) or extracellular spaces, thereby regulating zinc distribution and concentration within cells. Zinc, an essential micronutrient, plays critical roles in enzymatic function, signal transduction, and gene expression, making SLC30A4 vital for maintaining metabolic balance and cellular health.
The SLC30A4 gene is located on chromosome 15q21.1 in humans and encodes a protein with six transmembrane domains, characteristic of the ZnT family. It is highly expressed in tissues such as the mammary gland, brain, and pancreas, where zinc regulation is crucial for physiological processes like lactation, neurotransmission, and insulin secretion. Dysregulation of SLC30A4 has been implicated in several pathologies, including diabetes, neurodegenerative disorders, and cancer. For instance, mutations or altered expression of ZnT4 may disrupt zinc signaling, contributing to β-cell dysfunction in diabetes or promoting tumor progression through zinc-dependent pathways.
Recombinant SLC30A4 protein is engineered for in vitro studies to elucidate its structure-function relationships, transport mechanisms, and interactions with other proteins. Produced using expression systems like Escherichia coli or mammalian cell cultures, the recombinant form often includes tags (e.g., His-tag) for purification and detection. Research utilizing this protein has advanced understanding of zinc's role in cellular processes and disease, offering potential therapeutic targets. For example, modulating SLC30A4 activity could help address zinc deficiency-related disorders or counteract zinc toxicity in conditions like Alzheimer’s disease. Overall, SLC30A4 recombinant protein serves as a key tool in exploring zinc biology and its implications for human health.
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