| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC30A3 |
| Uniprot No | Q99726 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-388aa |
| 氨基酸序列 | MEPSPAAGGLETTRLVSPRDRGGAGGSLRLKSLFTEPSEPLPEESKPVEMPFHHCHRDPLPPPGLTPERLHARRQLYAACAVCFVFMAGEVVGGYLAHSLAIMTDAAHLLADVGSMMGSLFSLWLSTRPATRTMTFGWHRSETLGALASVVSLWMVTGILLYLAFVRLLHSDYHIEGGAMLLTASIAVCANLLMAFVLHQAGPPHSHGSRGAEYAPLEEGPEEPLPLGNTSVRAAFVHVLGDLLQSFGVLAASILIYFKPQYKAADPISTFLFSICALGSTAPTLRDVLRILMEGTPRNVGFEPVRDTLLSVPGVRATHELHLWALTLTYHVASAHLAIDSTADPEAVLAEASSRLYSRFGFSSCTLQVEQYQPEMAQCLRCQEPPQA |
| 预测分子量 | 41,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC30A3(ZnT3)重组蛋白的3篇代表性文献信息：
1. **标题**：*"Expression and Purification of Recombinant Human ZnT3 for Structural Studies"*
**作者**：Smith J, et al.
**摘要**：研究报道了人源SLC30A3(ZnT3)重组蛋白在大肠杆菌中的表达与纯化方法，并通过圆二色光谱验证其二级结构完整性，为后续锌转运机制研究提供基础。
2. **标题**：*"Functional Characterization of Recombinant ZnT3 in Vesicular Zinc Transport"*
**作者**：Lee H, et al.
**摘要**：利用哺乳动物细胞系统表达重组ZnT3蛋白，证实其在囊泡锌离子转运中的关键作用，并揭示其C端结构域对锌结合能力的调控。
3. **标题**：*"Cryo-EM Structure of the ZnT3 Transporter Reveals Zinc Coordination Sites"*
**作者**：Wang Y, et al.
**摘要**：通过冷冻电镜解析重组ZnT3蛋白的高分辨率结构，发现其跨膜锌离子通道的关键氨基酸残基，阐明锌离子选择性转运的分子机制。
4. **标题**：*"Role of Recombinant ZnT3 in Amyloid-β Aggregation in Alzheimer's Models"*
**作者**：Garcia R, et al.
**摘要**：在体外模型中验证重组ZnT3蛋白调控突触锌浓度，影响β淀粉样蛋白聚集，提示其在阿尔茨海默病病理中的潜在作用。
(注：以上文献为模拟示例，实际文献需通过PubMed/Google Scholar检索获取。)
**Background of SLC30A3 Recombinant Protein**
SLC30A3 (solute carrier family 30 member 3), also known as ZnT3. is a zinc transporter protein encoded by the *SLC30A3* gene in humans. It belongs to the SLC30 family of cation diffusion facilitators, which regulate cellular zinc homeostasis by mediating zinc efflux from the cytoplasm into intracellular compartments or extracellular spaces. ZnT3 is primarily expressed in the brain, particularly in synaptic vesicles of glutamatergic neurons, where it plays a critical role in loading zinc ions into vesicles for subsequent release during neurotransmission. This zincergic signaling is essential for modulating synaptic plasticity, memory formation, and neuronal communication.
The recombinant SLC30A3 protein is engineered to study its structure, function, and interactions in vitro. Produced through heterologous expression systems (e.g., bacterial, mammalian, or insect cells), the recombinant form often includes tags (e.g., His-tag) for purification and detection. Researchers use it to investigate zinc transport mechanisms, its role in neurological disorders, and potential therapeutic targets. For instance, dysregulation of ZnT3 has been linked to neurodegenerative diseases like Alzheimer’s, where zinc mislocalization may contribute to amyloid-beta aggregation. Additionally, studies explore its involvement in mood disorders and epilepsy, highlighting its broader physiological relevance.
Recombinant SLC30A3 also aids in developing assays for drug screening or identifying molecules that modulate zinc transport, offering insights into treating zinc-associated pathologies. Its study bridges molecular biology with neuroscience, emphasizing the importance of zinc homeostasis in health and disease.
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