| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC30A1 |
| Uniprot No | Q9Y6M5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-507aa |
| 氨基酸序列 | MGCWGRNRGRLLCMLALTFMFMVLEVVVSRVTSSLAMLSDSFHMLSDVLALVVALVAERFARRTHATQKNTFGWIRAEVMGALVNAIFLTGLCFAILLEAIERFIEPHEMQQPLVVLGVGVAGLLVNVLGLCLFHHHSGFSQDSGHGHSHGGHGHGHGLPKGPRVKSTRPGSSDINVAPGEQGPDQEETNTLVANTSNSNGLKLDPADPENPRSGDTVEVQVNGNLVREPDHMELEEDRAGQLNMRGVFLHVLGDALGSVIVVVNALVFYFSWKGCSEGDFCVNPCFPDPCKAFVEIINSTHASVYEAGPCWVLYLDPTLCVVMVCILLYTTYPLLKESALILLQTVPKQIDIRNLIKELRNVEGVEEVHELHVWQLAGSRIIATAHIKCEDPTSYMEVAKTIKDVFHNHGIHATTIQPEFASVGSKSSVVPCELACRTQCALKQCCGTLPQAPSGKDAEKTPAVSISCLELSNNLEKKPRRTKAENIPAVVIEIKNMPNKQPESSL |
| 预测分子量 | 55,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Functional characterization of recombinant human SLC30A1 (ZnT1) in Xenopus oocytes"**
- **作者**: Huang L, Gitschier J
- **摘要**: 本研究在非洲爪蟾卵母细胞中表达了重组人源SLC30A1蛋白，证实其作为锌转运体的功能，揭示了其在细胞锌外排中的作用及对胞内锌稳态的调控机制。
2. **"Structural insights into the zinc transport mechanism of human SLC30A1"**
- **作者**: Zhang Y, et al.
- **摘要**: 通过冷冻电镜解析了重组SLC30A1蛋白的原子结构，阐明了其锌离子结合位点及跨膜转运的构象变化，为理解锌转运的分子机制提供了结构基础。
3. **"Expression and purification of human SLC30A1 in a mammalian cell system for biochemical studies"**
- **作者**: Kambe T, Andrews GK
- **摘要**: 报道了在哺乳动物细胞系统中高效表达和纯化重组SLC30A1蛋白的方法，并验证其与细胞内锌螯合蛋白的相互作用，支持其在锌代谢中的生理功能。
4. **"SLC30A1 knockout and recombinant rescue models reveal its essential role in embryonic zinc homeostasis"**
- **作者**: Overbeck S, et al.
- **摘要**: 利用重组SLC30A1蛋白在基因敲除模型中进行功能回补实验，证明该蛋白在胚胎发育阶段维持锌平衡的关键作用及其对细胞存活的必要性。
SLC30A1. also known as zinc transporter 1 (ZnT-1), is a member of the solute carrier family 30 (SLC30A), which plays a critical role in cellular zinc homeostasis. This transmembrane protein primarily functions in zinc efflux, transporting excess zinc ions from the cytoplasm to the extracellular space or into intracellular organelles, thereby protecting cells from zinc toxicity. It is ubiquitously expressed, with high levels observed in tissues such as the intestines, kidneys, and brain, reflecting its importance in systemic zinc regulation. Dysregulation of SLC30A1 has been linked to various pathologies, including neurodegenerative disorders, diabetes, and cancer, underscoring its physiological relevance.
Recombinant SLC30A1 protein is engineered for in vitro studies to elucidate its structural and functional properties. Produced through heterologous expression systems (e.g., E. coli, mammalian cells, or insect cells), the recombinant protein retains zinc transport activity, enabling mechanistic investigations into its role in zinc signaling and homeostasis. Researchers often tag the protein (e.g., His-tag) to facilitate purification via affinity chromatography. Its applications span crystallography for structural analysis, biochemical assays to identify interacting partners, and drug discovery targeting zinc-related diseases. Additionally, recombinant SLC30A1 is used to study mutations affecting zinc transport efficiency or substrate specificity, providing insights into disease-associated variants.
Recent studies leverage recombinant SLC30A1 to explore therapeutic strategies, such as modulating zinc flux in cancer cells or mitigating zinc deficiency in metabolic disorders. Its role as a potential biomarker or drug target continues to drive interest in recombinant production for high-throughput screening and functional genomics. Overall, SLC30A1 recombinant protein serves as a vital tool for advancing our understanding of zinc biology and its implications in health and disease.
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