| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC26A4 |
| Uniprot No | O43511 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-780aa |
| 氨基酸序列 | MAAPGGRSEPPQLPEYSCSYMVSRPVYSELAFQQQHERRLQERKTLRESL AKCCSCSRKRAFGVLKTLVPILEWLPKYRVKEWLLSDVISGVSTGLVATL QGMAYALLAAVPVGYGLYSAFFPILTYFIFGTSRHISVGPFPVVSLMVGS VVLSMAPDEHFLVSSSNGTVLNTTMIDTAARDTARVLIASALTLLVGIIQ LIFGGLQIGFIVRYLADPLVGGFTTAAAFQVLVSQLKIVLNVSTKNYNGV LSIIYTLVEIFQNIGDTNLADFTAGLLTIVVCMAVKELNDRFRHKIPVPI PIEVIVTIIATAISYGANLEKNYNAGIVKSIPRGFLPPELPPVSLFSEML AASFSIAVVAYAIAVSVGKVYATKYDYTIDGNQEFIAFGISNIFSGFFSC FVATTALSRTAVQESTGGKTQVAGIISAAIVMIAILALGKLLEPLQKSVL AAVVIANLKGMFMQLCDIPRLWRQNKIDAVIWVFTCIVSIILGLDLGLLA GLIFGLLTVVLRVQFPSWNGLGSIPSTDIYKSTKNYKNIEEPQGVKILRF SSPIFYGNVDGFKKCIKSTVGFDAIRVYNKRLKALRKIQKLIKSGQLRAT KNGIISDAVSTNNAFEPDEDIEDLEELDIPTKEIEIQVDWNSELPVKVNV PKVPIHSLVLDCGAISFLDVVGVRSLRVIVKEFQRIDVNVYFASLQDYVI EKLEQCGFFDDNIRKDTFFLTVHDAILYLQNQVKSQEGQGSILETITLIQ DCKDTLELIETELTEEELDVQDEAMRTLAS |
| 预测分子量 | 113 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC26A4重组蛋白的模拟参考文献示例(内容为概括性描述，非真实文献)：
1. **《Functional Characterization of Recombinant SLC26A4/Pendrin in Epithelial Ion Transport》**
- 作者：Smith J, et al.
- 摘要：研究利用HEK293细胞表达重组SLC26A4蛋白，证实其作为氯离子/碘离子转运体的功能，并发现pH值变化显著影响其活性。
2. **《Structural Insights into SLC26A4 Mutations Associated with Hearing Loss》**
- 作者：Wang L, et al.
- 摘要：通过冷冻电镜解析重组SLC26A4的晶体结构，揭示常见致病突变(如L236P)导致蛋白构象异常，破坏跨膜转运机制。
3. **《In Vitro Reconstitution of Pendrin-Mediated Bicarbonate Secretion》**
- 作者：Kim H, et al.
- 摘要：在人工脂质体中重构重组SLC26A4蛋白，证明其在碳酸氢盐分泌中的关键作用，并验证与FOXI1转录因子的协同调控。
4. **《High-Throughput Screening for SLC26A4 Modulators Using a Fluorescent Assay》**
- 作者：Garcia R, et al.
- 摘要：开发基于重组SLC26A4的荧光标记方法，筛选小分子库发现两种化合物可恢复致病突变体的离子转运功能。
(注：以上为示例性内容，实际文献需通过PubMed/Google Scholar等平台检索确认。)
**Background of SLC26A4 Recombinant Protein**
The SLC26A4 gene encodes pendrin, a transmembrane anion transporter belonging to the solute carrier 26 (SLC26) family. This protein plays a critical role in ion homeostasis, particularly in the inner ear, thyroid, and kidneys. Pendrin facilitates the exchange of chloride, iodide, bicarbonate, and other anions, contributing to processes like endolymphatic fluid pH regulation in the cochlea and iodide organification in the thyroid.
Mutations in SLC26A4 are linked to Pendred syndrome, a genetic disorder characterized by sensorineural hearing loss, vestibular dysfunction, and thyroid goiter. Studying the protein's structure and function is essential for understanding disease mechanisms. Recombinant SLC26A4 protein, produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), allows researchers to analyze its transport activity, post-translational modifications, and interactions in controlled environments.
The production of recombinant SLC26A4 faces challenges due to its complex membrane-bound structure and the need for proper folding. Advances in structural biology, such as cryo-EM, have begun to elucidate its conformational dynamics. Recombinant variants are also used to test therapeutic strategies, including pharmacological chaperones to rescue misfolded mutants in Pendred syndrome.
Current research focuses on clarifying pendrin’s role in non-syndromic hearing loss, thyroid disorders, and its potential interactions with other ion transporters. Recombinant SLC26A4 remains a vital tool for dissecting pathophysiological pathways and developing targeted treatments for related diseases.
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