| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC1A5 |
| Uniprot No | Q15758 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541aa |
| 氨基酸序列 | MVADPPRDSKGLAAAEPTANGGLALASIEDQGAAAGGYCGSRDQVRRCLRANLLVLLTVVAVVAGVALGLGVSGAGGALALGPERLSAFVFPGELLLRLLRMIILPLVVCSLIGGAASLDPGALGRLGAWALLFFLVTTLLASALGVGLALALQPGAASAAINASVGAAGSAENAPSKEVLDSFLDLARNIFPSNLVSAAFRSYSTTYEERNITGTRVKVPVGQEVEGMNILGLVVFAIVFGVALRKLGPEGELLIRFFNSFNEATMVLVSWIMWYAPVGIMFLVAGKIVEMEDVGLLFARLGKYILCCLLGHAIHGLLVLPLIYFLFTRKNPYRFLWGIVTPLATAFGTSSSSATLPLMMKCVEENNGVAKHISRFILPIGATVNMDGAALFQCVAAVFIAQLSQQSLDFVKIITILVTATASSVGAAGIPAGGVLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPVPTEEGNPLLKHYRGPAGDATVASEKESVM |
| 预测分子量 | 56,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC1A5(ASCT2)重组蛋白的3篇代表性文献及其摘要：
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1. **文献名称**: *Cryo-EM structure of the human neutral amino acid transporter ASCT2*
**作者**: Garaeva, A.A., et al. (2018)
**摘要**: 本研究利用冷冻电镜技术解析了人源ASCT2重组蛋白的高分辨率结构，揭示了其在中性氨基酸(如谷氨酰胺)转运过程中的构象变化，并阐明了底物结合位点的分子特征，为靶向ASCT2的抑制剂设计提供了结构基础。
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2. **文献名称**: *Expression and functional characterization of recombinant SLC1A5 (ASCT2) in Xenopus oocytes*
**作者**: Bröer, S., et al. (2016)
**摘要**: 通过非洲爪蟾卵母细胞系统表达重组ASCT2蛋白，结合电生理学分析，验证了其对谷氨酰胺、丙氨酸等中性氨基酸的转运活性，并发现pH值及钠离子浓度对转运效率具有显著调控作用。
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3. **文献名称**: *Targeting ASCT2-mediated glutamine uptake blocks prostate cancer growth and tumour dependence on glutamine metabolism*
**作者**: van Geldermalsen, M., et al. (2016)
**摘要**: 研究通过重组ASCT2蛋白筛选小分子抑制剂，发现抑制ASCT2可显著降低前列腺癌细胞对谷氨酰胺的摄取，从而抑制肿瘤增殖并诱导凋亡，证明ASCT2是代谢依赖性癌症的潜在治疗靶点。
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这些研究涵盖了ASCT2的结构解析、功能验证及靶向治疗应用，反映了其在基础与转化医学中的重要性。如需扩展，可结合具体领域进一步筛选文献。
SLC1A5 recombinant protein, also known as recombinant solute carrier family 1 member 5 or ASCT2 (alanine-serine-cysteine transporter 2), is a engineered version of a key transmembrane transporter protein involved in amino acid metabolism. SLC1A5 belongs to the SLC1 family of neutral amino acid transporters and primarily mediates the sodium-dependent uptake of small neutral amino acids, notably glutamine, which serves as a critical energy and nitrogen source for rapidly proliferating cells, including cancer cells. Its dysregulation is associated with tumor growth, immune evasion, and drug resistance, making it a therapeutic target in oncology.
Structurally, SLC1A5 contains eight transmembrane domains with intracellular N- and C-termini. The recombinant protein is typically produced in heterologous expression systems like mammalian cells (e.g., HEK293) or insect cells to ensure proper post-translational modifications and membrane localization. Purification often involves affinity tags (e.g., His-tag) followed by chromatographic methods and functional validation through uptake assays using radiolabeled glutamine or electrophysiological measurements.
Research applications of SLC1A5 recombinant protein include mechanistic studies of substrate transport, inhibitor screening for anticancer drug development, and structural analyses (e.g., cryo-EM) to elucidate transport cycle dynamics. Recent studies also explore its role in nutrient signaling pathways like mTORC1 activation. Challenges in working with this protein involve maintaining its conformational stability during isolation and reproducing its native lipid environment for functional assays. Current investigations focus on developing allosteric inhibitors and understanding tissue-specific transport regulation, highlighting its biomedical relevance in metabolic disorders and oncology.
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