| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COMMD9 |
| Uniprot No | Q9P000 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAALTAEHFAALQSLLKASSKDVVRQL CQESFSSSALGLKKLLDVTCSSLSVTQEEAEELLQALHRLTRLVAFRDLS SAEAILALFPENFHQNLKNLLTKIILEHVSTWRTEAQANQISLPRLVDLD WRVDIKTSSDSISRMAVPTCLLQMKIQEDPSLCGDKPSISAVTVELSKET LDTMLDGLGRIRDQLSAVASK |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COMMD9重组蛋白的3篇示例参考文献(注：以下内容为假设性示例，实际文献需根据具体研究补充)：
1. **文献名称**：*COMMD9 interacts with the C-terminal domain of NF-κB1/p105 and regulates its nuclear translocation*
**作者**：Zhang L, et al.
**摘要**：本研究利用重组COMMD9蛋白进行体外结合实验，发现其通过C端结构域直接结合NF-κB1/p105.抑制其核转位并下调炎症反应，揭示了COMMD9在NF-κB信号通路中的调控机制。
2. **文献名称**：*Recombinant COMMD9 suppresses tumor growth by modulating copper metabolism in colorectal cancer*
**作者**：Wang Y, et al.
**摘要**：通过表达纯化人源COMMD9重组蛋白，研究发现其可结合铜转运蛋白ATP7A，降低细胞内铜离子浓度，从而抑制结直肠癌细胞增殖并诱导凋亡，提示其在癌症治疗中的潜在应用。
3. **文献名称**：*Structural characterization of COMMD9 and its interaction with CCDC22 using recombinant protein technology*
**作者**：Chen R, et al.
**摘要**：本研究解析了重组COMMD9蛋白的晶体结构，并利用表面等离子共振(SPR)技术证实其与CCDC22的相互作用，揭示了COMMD9-CCDC22复合物在内体运输中的功能。
(注：实际文献需通过PubMed/Google Scholar等平台以“COMMD9 recombinant protein”为关键词检索。)
**Background of COMMD9 Recombinant Protein**
COMMD9 (COMM domain-containing protein 9) is a member of the COMMD protein family, which comprises 10 conserved members (COMMD1-10) sharing a characteristic C-terminal COMM domain. This domain facilitates protein-protein interactions and is implicated in diverse cellular processes, including transcriptional regulation, ion transport, protein trafficking, and degradation. COMMD9. specifically, has garnered attention for its role in modulating endosomal sorting and cellular signaling pathways, particularly through interactions with the copper metabolism Murr1 (COMMD) family and other partners like CCDC22.
Studies suggest COMMD9 participates in the regulation of the epidermal growth factor receptor (EGFR) pathway by influencing receptor ubiquitination and lysosomal degradation, impacting cell proliferation and cancer progression. Dysregulation of COMMD9 has been linked to malignancies such as breast and colorectal cancers, highlighting its potential as a therapeutic target. Additionally, COMMD9 may interact with NF-κB signaling, suggesting roles in inflammation and immune responses.
Recombinant COMMD9 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, biologically active protein for functional studies. Its recombinant form enables researchers to investigate molecular mechanisms, protein interactions, and enzymatic activities *in vitro* or in cell-based assays. Applications include elucidating COMMD9's role in endosomal sorting complexes, cancer biology, and immune regulation, as well as developing diagnostic or therapeutic agents.
Overall, COMMD9 recombinant protein serves as a critical tool for decoding its physiological and pathological functions, bridging gaps between cellular biochemistry and disease mechanisms.
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