| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHD5 |
| Uniprot No | Q8TDI0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHD5重组蛋白的3篇示例参考文献(注：以下为模拟示例，非真实文献)：
1. **文献名称**："CHD5 Recombinant Protein Suppresses Tumor Growth via Chromatin Remodeling in Neuroblastoma"
**作者**：Smith J, et al.
**摘要**：该研究通过体外表达和纯化CHD5重组蛋白，证明其在神经母细胞瘤细胞中通过重塑染色质结构抑制致癌基因表达，显著抑制肿瘤细胞增殖并诱导凋亡。
2. **文献名称**："Functional Characterization of CHD5 Recombinant Protein in Neuronal Differentiation"
**作者**：Lee S, et al.
**摘要**：文章利用大肠杆菌系统表达CHD5重组蛋白，发现其能够结合神经元特异性基因启动子区域，促进胚胎干细胞向神经元分化，揭示了CHD5在神经发育中的表观遗传调控机制。
3. **文献名称**："Structural Insights into CHD5-DNA Interaction Using Recombinant Protein Crystallography"
**作者**：Wang Y, et al.
**摘要**：本研究通过X射线晶体学解析CHD5重组蛋白与DNA复合物的三维结构，阐明了其双PHD锌指结构域特异性识别组蛋白修饰H3K4me3的分子机制，为开发靶向CHD5的癌症疗法提供结构基础。
CHD5 (Chromodomain Helicase DNA-Binding Protein 5) is a member of the CHD family of chromatin-remodeling proteins, which play critical roles in regulating gene expression by altering chromatin structure. Discovered in 2003. CHD5 is encoded by the *CHD5* gene located on chromosome 1p36.3. a region frequently deleted in human cancers. It contains conserved domains, including chromodomains for methylated histone recognition, a helicase ATP-binding domain for chromatin remodeling, and a DNA-binding domain, enabling its function in transcriptional regulation and genome stability maintenance.
CHD5 acts as a tumor suppressor, particularly in neural and epithelial tissues. It recruits chromatin-modifying complexes to promote differentiation and inhibit proliferation by repressing oncogenic pathways or activating tumor-suppressive genes. Its loss or mutation is linked to neuroblastoma, glioblastoma, and other cancers, correlating with poor prognosis. Beyond oncology, CHD5 is essential in neurodevelopment, influencing synaptic plasticity and neuronal survival.
Recombinant CHD5 protein, produced via bacterial or mammalian expression systems, is a key tool for studying its biochemical properties, interactions, and mechanisms. Researchers use it to investigate chromatin remodeling dynamics, histone modification crosstalk, and DNA repair processes. It also aids in screening therapeutic compounds targeting CHD5-deficient cancers or neurodevelopmental disorders. However, producing full-length functional CHD5 remains challenging due to its large size (~200 kDa) and post-translational modification dependencies. Current efforts focus on optimizing expression systems and resolving structural details to advance its therapeutic applications. As a biomarker or drug target, CHD5 holds promise for precision medicine in cancer and neurological diseases.
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