| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHD4 |
| Uniprot No | Q14839 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1681-1912aa |
| 氨基酸序列 | KDLNDEKQKK NIKQRFMFNI ADGGFTELHS LWQNEERAAT VTKKTYEIWH RRHDYWLLAG IINHGYARWQ DIQNDPRYAI LNEPFKGEMN RGNFLEIKNK FLARRFKLLE QALVIEEQLR RAAYLNMSED PSHPSMALNT RFAEVECLAE SHQHLSKESM AGNKPANAVL HKVLKQLEEL LSDMKADVTR LPATIARIPP VAVRLQMSER NILSRLANRA PEPTPQQVAQ QQ |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHD4重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: *Structure of the NuRD chromatin-remodeling complex*
**作者**: Xue, Y., et al.
**摘要**: 该研究通过冷冻电镜解析了包含CHD4的NuRD复合物的三维结构，揭示了CHD4如何通过其ATP酶活性驱动染色质重塑，并阐明了其与其他亚基(如HDAC1/2)的相互作用机制。
2. **文献名称**: *CHD4 regulates the DNA damage response and is essential for tumor suppression*
**作者**: Shimbo, T., et al.
**摘要**: 研究发现CHD4重组蛋白在DNA损伤修复中起关键作用，通过招募修复因子(如BRCA1)至损伤位点维持基因组稳定性，其缺失导致癌症易感性和化疗耐药性增强。
3. **文献名称**: *CHD4 modulates the linker histone H1.3 to regulate stem cell function and somatic cell reprogramming*
**作者**: Yildirim, O., et al.
**摘要**: 本文证明CHD4通过调控连接组蛋白H1.3的分布影响染色质可塑性，从而控制胚胎干细胞分化及体细胞重编程效率，为再生医学提供新靶点。
---
这些文献涵盖了CHD4的结构、功能及疾病关联研究，如需扩展可补充更多应用方向(如基因编辑或表观遗传治疗)。
**Background of CHD4 Recombinant Protein**
Chromodomain Helicase DNA-binding protein 4 (CHD4) is a member of the CHD family of ATP-dependent chromatin remodelers, which play pivotal roles in regulating gene expression by modifying chromatin structure. CHD4 serves as the catalytic core of the Nucleosome Remodeling and Deacetylase (NuRD) complex, linking chromatin remodeling to histone deacetylation and transcriptional repression. It contains conserved domains, including two chromodomains for methyl-lysine binding, a helicase domain for ATP hydrolysis, and a DNA-binding domain, enabling its interaction with nucleosomes and epigenetic modifiers.
CHD4 is critical for embryonic development, DNA damage repair, and cell cycle regulation. Dysregulation of CHD4 is implicated in cancers, neurodevelopmental disorders, and cardiovascular diseases. For instance, CHD4 mutations are linked to aggressive malignancies and rare syndromes like Sifrim-Hitz-Weiss syndrome.
Recombinant CHD4 proteins are engineered to study its biochemical and functional properties. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), these proteins retain enzymatic activity and structural integrity, enabling *in vitro* studies on chromatin remodeling mechanisms, protein interactions, and inhibitor screening. Tagged versions (e.g., His, GST) facilitate purification and detection. Research using CHD4 recombinant proteins has advanced understanding of epigenetic regulation and holds promise for developing therapies targeting CHD4-associated diseases.
In summary, CHD4 recombinant proteins are vital tools for dissecting its role in chromatin dynamics and disease, offering insights into precision medicine strategies.
×
