| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHD3 |
| Uniprot No | Q12873 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHD3重组蛋白的模拟参考文献示例(注：内容为虚构，仅供格式参考)：
1. **"Purification and functional characterization of recombinant CHD3 chromatin remodeler"**
- **作者**: Smith A, et al.
- **摘要**: 本研究利用杆状病毒-昆虫细胞系统表达并纯化重组CHD3蛋白，证实其具有ATP依赖的染色质重塑活性，并揭示了其通过解旋酶结构域与核小体DNA的相互作用机制。
2. **"Structural insights into the nucleosome recognition by CHD3 ATPase domain"**
- **作者**: Lee J, et al.
- **摘要**: 通过冷冻电镜解析重组CHD3蛋白ATP酶结构域与核小体的复合物结构，揭示了CHD3特异性识别组蛋白H3尾部修饰的分子基础，为染色质重塑机制提供新见解。
3. **"CHD3 recombinant protein suppresses tumor growth by modulating oncogenic transcription networks"**
- **作者**: Chen L, et al.
- **摘要**: 在大肠杆菌中表达重组CHD3蛋白，发现其通过结合特定启动子区域抑制癌基因MYC的转录，并在体外细胞模型中验证其抗肿瘤活性。
4. **"Development of a high-throughput assay for CHD3 helicase activity using recombinant protein"**
- **作者**: Gupta R, et al.
- **摘要**: 建立基于荧光共振能量转移(FRET)的CHD3解旋酶活性检测方法，利用重组蛋白筛选小分子抑制剂，为靶向染色质重塑的疾病治疗提供工具。
建议通过PubMed、Google Scholar等平台检索真实文献，关键词："CHD3 recombinant protein"、"CHD3 purification/function/structure"。
**Background of CHD3 Recombinant Protein**
CHD3 (Chromodomain Helicase DNA-binding protein 3) is a member of the CHD family of chromatin-remodeling proteins, which play critical roles in regulating gene expression by modifying chromatin structure. These proteins utilize ATP hydrolysis to reposition nucleosomes, thereby influencing DNA accessibility for transcription, replication, and repair. CHD3 is specifically associated with the NuRD (Nucleosome Remodeling and Deacetylase) complex, linking chromatin remodeling to histone deacetylase activity for transcriptional repression.
The CHD3 protein contains conserved domains, including chromodomains that recognize methylated histones, a helicase-like ATPase domain for chromatin remodeling, and DNA-binding motifs. Dysregulation of CHD3 has been implicated in developmental disorders, cancers, and neurological diseases, underscoring its importance in cellular homeostasis.
Recombinant CHD3 protein is engineered for *in vitro* studies to dissect its biochemical and functional properties. Produced using expression systems like *E. coli* or mammalian cell lines, it is purified via affinity tags (e.g., His-tag) and validated for ATPase activity, nucleosome remodeling, and interaction with NuRD components. Researchers employ recombinant CHD3 to study its role in epigenetic regulation, DNA repair mechanisms, and disease pathways. It also serves as a tool for screening potential therapeutic compounds targeting chromatin remodeling in cancers or genetic disorders.
Current challenges include maintaining the protein’s native conformation and enzymatic activity post-purification. Advances in structural biology and CRISPR-based functional assays using recombinant CHD3 continue to shed light on its molecular mechanisms, offering insights into novel epigenetic therapies.
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