| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HCCS |
| Uniprot No | P53701 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-268aa |
| 氨基酸序列 | MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS |
| 预测分子量 | 57.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HCCS重组蛋白的参考文献示例(注：部分文献为假设性示例，实际引用时需核实真实来源)：
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1. **标题**: *Recombinant Expression and Functional Analysis of Human Holocytochrome c Synthase in Escherichia coli*
**作者**: Smith J, et al.
**摘要**: 本研究报道了人源HCCS在大肠杆菌中的重组表达及纯化，证实其通过催化血红素与脱辅基细胞色素c的结合，促进功能性细胞色素c的生成，为体外研究线粒体电子传递链蛋白成熟机制提供了工具。
2. **标题**: *Structural Insights into HCCS-Mediated Heme Ligation during Cytochrome c Maturation*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析重组HCCS蛋白的三维结构，揭示了其与血红素及脱辅基细胞色素c的相互作用位点，阐明了HCCS在血红素共价连接中的催化机制。
3. **标题**: *Defective HCCS Activity in Mitochondrial Disorders: Rescue by Recombinant Protein Supplementation*
**作者**: Garcia R, et al.
**摘要**: 研究证明HCCS基因突变导致的重组蛋白功能缺失与微缺失综合征相关，体外实验表明外源性重组HCCS可部分恢复患者细胞中细胞色素c的成熟，提示潜在治疗策略。
4. **标题**: *Optimization of HCCS Recombinant Production in Insect Cells for Enzymatic Assays*
**作者**: Brown K, et al.
**摘要**: 开发了基于杆状病毒表达系统的HCCS重组蛋白高效生产与纯化流程，并建立体外酶活检测体系，为高通量筛选HCCS抑制剂或激活剂奠定基础。
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**注意**：以上文献为示例性质，实际引用时需通过学术数据库(如PubMed、Web of Science)检索真实发表的研究，并核对作者、标题及摘要准确性。建议使用关键词“HCCS recombinant”“holocytochrome c synthase expression”进行扩展搜索。
**Background of HCCS Recombinant Protein**
Holocytochrome c synthase (HCCS) is a mitochondrial enzyme critical for the biosynthesis of cytochrome c, a key protein involved in electron transport during cellular respiration and apoptosis regulation. HCCS catalyzes the covalent attachment of heme to the apoprotein of cytochrome c, a post-translational modification essential for its functional maturation. Defects in HCCS activity are linked to mitochondrial disorders, including microphthalmia with linear skin lesions (MLS) syndrome, underscoring its biological importance.
Recombinant HCCS protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable large-scale production for research and therapeutic applications. Its recombinant form retains enzymatic activity, allowing in vitro studies to dissect heme attachment mechanisms, cytochrome c biogenesis, and mitochondrial dysfunction. Researchers utilize HCCS recombinant protein to model mitochondrial diseases, screen potential drugs targeting cytochrome c-related pathways, and explore apoptotic signaling cascades in cancer biology.
Structural studies of recombinant HCCS have provided insights into its interaction with substrates and regulatory cofactors. Additionally, it serves as a tool for developing gene therapies for HCCS-linked genetic disorders. The ability to produce pure, functional HCCS in vitro has accelerated both basic mitochondrial research and translational efforts to address cytochrome c deficiency syndromes.
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