| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CX3CR1 |
| Uniprot No | P49238 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-355aa |
| 氨基酸序列 | MDQFPESVTENFEYDDLAEACYIGDIVVFGTVFLSIFYSVIFAIGLVGNL LVVFALTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNA MCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTIS LGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGF LLPLLIMSYCYFRIIQTLFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVM IFLETLKLYDFFPSCDMRKDLRLALSVTETVAFSHCCLNPLIYAFAGEKF RRYLYHLYGKCLAVLCGRSVHVDFSSSESQRSRHGSVLSSNFTYHTSDGD ALLLL |
| 预测分子量 | 65 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CX3CR1重组蛋白的参考文献示例(注：文献标题和作者为虚构示例，实际文献需通过数据库验证)：
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1. **标题**: *Structural basis of CX3CL1 recognition by the chemokine receptor CX3CR1*
**作者**: Zhang Y. et al. (2021)
**摘要**: 本研究通过表达重组人源CX3CR1蛋白，结合冷冻电镜技术解析了其与配体CX3CL1(fractalkine)结合的复合物结构，揭示了受体跨膜区关键氨基酸与配体的相互作用机制，为靶向CX3CR1的药物设计提供了结构基础。
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2. **标题**: *Recombinant CX3CR1 mediates microglial migration in Alzheimer's disease models*
**作者**: Rogers J. et al. (2019)
**摘要**: 利用HEK293细胞表达重组CX3CR1蛋白，研究其在β-淀粉样蛋白激活小胶质细胞迁移中的作用，发现CX3CR1/CX3CL1信号轴通过激活MAPK通路促进神经炎症，提示其在阿尔茨海默病中的潜在治疗靶点。
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3. **标题**: *Functional characterization of CX3CR1 mutants using recombinant protein binding assays*
**作者**: Fong A.M. et al. (2016)
**摘要**: 通过昆虫细胞系统表达并纯化重组CX3CR1蛋白及突变体，结合表面等离子体共振(SPR)技术定量分析其与CX3CL1的亲和力变化，确定了受体N端结构域对配体结合的关键作用。
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**建议**：实际文献可通过PubMed或Google Scholar检索关键词“CX3CR1 recombinant protein”或“CX3CR1 structure/function”获取，重点关注近年发表的蛋白质结构、信号机制或疾病相关研究。
CX3CR1. also known as the C-X3-C motif chemokine receptor 1. is a class A G protein-coupled receptor (GPCR) primarily expressed on immune cells such as monocytes, macrophages, microglia, and natural killer (NK) cells. It binds specifically to its sole ligand, CX3CL1 (fractalkine), a unique transmembrane chemokine that exists in both soluble and membrane-bound forms. The CX3CR1-CX3CL1 axis plays critical roles in immune surveillance, cell adhesion, and migration, particularly in inflammatory and neurodegenerative conditions.
Structurally, CX3CR1 contains seven transmembrane domains and multiple post-translational modification sites, including glycosylation and phosphorylation motifs. Its interaction with CX3CL1 triggers intracellular signaling pathways involving G proteins, β-arrestins, and downstream effectors, regulating chemotaxis, cell survival, and immune responses. Research has linked CX3CR1 polymorphisms to altered disease susceptibility, including Alzheimer’s disease, atherosclerosis, and HIV progression, due to its role in modulating microglial activation and leukocyte trafficking.
Recombinant CX3CR1 protein is produced in vitro using expression systems (e.g., mammalian or insect cells) to preserve native conformational epitopes and post-translational modifications. This engineered protein retains ligand-binding capability and signaling functions, making it valuable for studying receptor-ligand interactions, screening therapeutic agents targeting inflammatory or neurological disorders, and developing monoclonal antibodies. In drug discovery, CX3CR1 inhibitors are explored for treating chronic inflammation, while its role in tumor microenvironments has spurred interest in cancer immunotherapy. The protein’s stability and functional consistency in recombinant form have also facilitated structural studies using cryo-EM and X-ray crystallography, advancing GPCR biology research.
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