| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCR4 |
| Uniprot No | P51679 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-360aa |
| 氨基酸序列 | MNPTDIADTTLDESIYSNYYLYESIPKPCTKEGIKAFGELFLPPLYSLVF VFGLLGNSVVVLVLFKYKRLRSMTDVYLLNLAISDLLFVFSLPFWGYYAA DQWVFGLGLCKMISWMYLVGFYSGIFFVMLMSIDRYLAIVHAVFSLRART LTYGVITSLATWSVAVFASLPGFLFSTCYTERNHTYCKTKYSLNSTTWKV LSSLEINILGLVIPLGIMLFCYSMIIRTLQHCKNEKKNKAVKMIFAVVVL FLGFWTPYNIVLFLETLVELEVLQDCTFERYLDYAIQATETLAFVHCCLN PIIYFFLGEKFRKYILQLFKTCRGLFVLCQYCGLLQIYSADTPSSSYTQS TMDHDLHDAL |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCR4重组蛋白研究的示例参考文献(注：以下文献为示例，具体文献请通过学术数据库查询)：
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1. **文献名称**：*Structural characterization of recombinant human CCR4 chemokine receptor*
**作者**：Smith A, et al.
**摘要**：本研究通过杆状病毒-昆虫细胞系统成功表达并纯化了重组人CCR4蛋白，利用冷冻电镜技术解析了其三维结构，揭示了其与趋化因子CCL17结合的分子机制，为靶向CCR4的药物设计提供了结构基础。
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2. **文献名称**：*Functional analysis of CCR4 in T-cell migration using recombinant protein models*
**作者**：Li Y, Tanaka K.
**摘要**：通过哺乳动物细胞表达重组CCR4蛋白，研究其在T细胞趋化运动中的作用。实验表明，CCR4与CCL22的结合可激活下游信号通路，并促进调节性T细胞(Tregs)向炎症部位的迁移。
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3. **文献名称**：*Development of a CCR4-based biosensor for high-throughput drug screening*
**作者**：Garcia R, et al.
**摘要**：构建了基于重组CCR4蛋白的生物传感器，结合表面等离子体共振(SPR)技术，筛选出多种小分子抑制剂，验证了CCR4作为癌症免疫治疗靶点的潜力。
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4. **文献名称**：*Expression optimization and functional reconstitution of CCR4 in lipid bilayers*
**作者**：Wang X, et al.
**摘要**：通过优化大肠杆菌表达系统获得高纯度重组CCR4蛋白，并将其成功重组至脂质双分子层中，证实其在非天然膜环境下的配体结合活性，为体外功能研究提供新方法。
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**建议**：
可通过 **PubMed**、**Web of Science** 或 **Google Scholar** 检索关键词 *"CCR4 recombinant protein"*、*"CCR4 expression and purification"* 获取最新研究。例如：
- 真实文献可能包括 *Nature Structural & Molecular Biology* 或 *Journal of Biological Chemistry* 等期刊中的相关论文。
CCR4 (C-C chemokine receptor type 4) is a G protein-coupled receptor (GPCR) predominantly expressed on immune cells, including T-helper type 2 (Th2) cells, regulatory T cells (Tregs), and dendritic cells. It plays a critical role in mediating immune responses by binding to specific chemokines, notably CCL17 (TARC) and CCL22 (MDC). These ligand-receptor interactions guide leukocyte migration to sites of inflammation, influencing allergic responses, tumor microenvironments, and autoimmune disorders. Dysregulation of CCR4 is implicated in pathologies such as asthma, atopic dermatitis, and cancer metastasis.
Recombinant CCR4 protein is engineered in vitro using expression systems like mammalian or insect cells to preserve post-translational modifications and structural integrity. It typically includes tags (e.g., His-tag) for purification and detection. This protein serves as a vital tool for studying CCR4-ligand binding kinetics, signal transduction pathways (e.g., MAPK/ERK, PI3K/Akt), and receptor internalization mechanisms. Researchers also use it to screen therapeutic agents targeting CCR4. including monoclonal antibodies (e.g., mogamulizumab for T-cell lymphomas) and small-molecule inhibitors for inflammatory diseases. Additionally, it aids in developing diagnostic assays to quantify CCR4 expression in clinical samples. Its applications extend to elucidating CCR4's role in HIV co-receptor activity and Treg-mediated immune tolerance.
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