| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KTN1 |
| Uniprot No | Q86UP2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1357aa |
| 氨基酸序列 | MEFYESAYFIVLIPSIVITVIFLFFWLFMKETLYDEVLAKQKREQKLIPTKTDKKKAEKKKNKKKEIQNGNLHESDSESVPRDFKLSDALAVEDDQVAPVPLNVVETSSSVRERKKKEKKQKPVLEEQVIKESDASKIPGKKVEPVPVTKQPTPPSEAAASKKKPGQKKSKNGSDDQDKKVETLMVPSKRQEALPLHQETKQESGSGKKKASSKKQKTENVFVDEPLIHATTYIPLMDNADSSPVVDKREVIDLLKPDQVEGIQKSGTKKLKTETDKENAEVKFKDFLLSLKTMMFSEDEALCVVDLLKEKSGVIQDALKKSSKGELTTLIHQLQEKDKLLAAVKEDAAATKDRCKQLTQEMMTEKERSNVVITRMKDRIGTLEKEHNVFQNKIHVSYQETQQMQMKFQQVREQMEAEIAHLKQENGILRDAVSNTTNQLESKQSAELNKLRQDYARLVNELTEKTGKLQQEEVQKKNAEQAATQLKVQLQEAERRWEEVQSYIRKRTAEHEAAQQDLQSKFVAKENEVQSLHSKLTDTLVSKQQLEQRLMQLMESEQKRVNKEESLQMQVQDILEQNEALKAQIQQFHSQIAAQTSASVLAEELHKVIAEKDKQIKQTEDSLASERDRLTSKEEELKDIQNMNFLLKAEVQKLQALANEQAAAAHELEKMQQSVYVKDDKIRLLEEQLQHEISNKMEEFKILNDQNKALKSEVQKLQTLVSEQPNKDVVEQMEKCIQEKDEKLKTVEELLETGLIQVATKEEELNAIRTENSSLTKEVQDLKAKQNDQVSFASLVEELKKVIHEKDGKIKSVEELLEAELLKVANKEKTVQDLKQEIKALKEEIGNVQLEKAQQLSITSKVQELQNLLKGKEEQMNTMKAVLEEKEKDLANTGKWLQDLQEENESLKAHVQEVAQHNLKEASSASQFEELEIVLKEKENELKRLEAMLKERESDLSSKTQLLQDVQDENKLFKSQIEQLKQQNYQQASSFPPHEELLKVISEREKEISGLWNELDSLKDAVEHQRKKNNDLREKNWEAMEALASTEKMLQDKVNKTSKERQQQVEAVELEAKEVLKKLFPKVSVPSNLSYGEWLHGFEKKAKECMAGTSGSEEVKVLEHKLKEADEMHTLLQLECEKYKSVLAETEGILQKLQRSVEQEENKWKVKVDESHKTIKQMQSSFTSSEQELERLRSENKDIENLRREREHLEMELEKAEMERSTYVTEVRELKDLLTELQKKLDDSYSEAVRQNEELNLLKAQLNETLTKLRTEQNERQKVAGDLHKAQQSLELIQSKIVKAAGDTTVIENSDVSPETESSEKETMSVSLNQTVTQLQQLLQAVNQQLTKEKEHYQVLE |
| 预测分子量 | 156,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KTN1重组蛋白的3篇参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant Human KTN1 in Mammalian Cells"*
**作者**：Chen L., Wang Y., et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了重组人源KTN1蛋白，并验证其与驱动蛋白KIF5B的相互作用。实验表明，重组KTN1在细胞内运输过程中通过调控微管网络动态影响囊泡转运效率。
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2. **文献名称**：*"Structural Analysis of KTN1 Reveals Its Role in ER Membrane Organization"*
**作者**：Saito M., Tanaka R., et al.
**摘要**：通过大肠杆菌表达系统获得高纯度重组KTN1蛋白，结合冷冻电镜技术解析其三维结构。研究发现，KTN1通过特定结构域锚定内质网膜，并参与维持内质网形态及膜蛋白分布。
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3. **文献名称**：*"Recombinant KTN1 as a Novel Biomarker for Neurodegenerative Disease Screening"*
**作者**：Gupta S., Patel T., et al.
**摘要**：利用重组KTN1蛋白开发ELISA检测方法，发现阿尔茨海默病患者脑脊液中KTN1水平显著异常。研究提示重组KTN1可作为神经退行性疾病的新型分子标记物。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“recombinant KTN1 protein”或“KTN1 expression and function”。
**Background of KTN1 Recombinant Protein**
KTN1 (Kinectin 1) is a cytosolic protein encoded by the *KTN1* gene, primarily known for its role in intracellular membrane trafficking and organelle movement. It serves as a receptor for kinesin-1. a motor protein involved in transporting vesicles, organelles, and protein complexes along microtubules. KTN1 is critical for maintaining the structural integrity of the endoplasmic reticulum (ER) and Golgi apparatus, facilitating cargo transport between these compartments and the plasma membrane.
The protein features a large coiled-coil structure, enabling interactions with multiple partners, including cytoskeletal components and signaling molecules. Its involvement in cellular processes like autophagy, cell adhesion, and receptor trafficking has linked KTN1 to various physiological and pathological conditions, such as cancer metastasis, neurodegenerative diseases, and immune responses. Dysregulation of KTN1 expression has been observed in tumors, where it may influence cell motility and invasiveness.
Recombinant KTN1 protein is typically produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications. This engineered protein retains functional domains, allowing researchers to study its biochemical interactions, structural dynamics, and role in disease mechanisms. Tagged versions (e.g., His-tag, GFP-tag) are commonly generated for purification and visualization in assays like pull-down experiments, immunofluorescence, or in vitro motility studies.
KTN1 recombinant tools are pivotal for elucidating trafficking pathways, screening therapeutic targets, and developing strategies to modulate kinesin-mediated transport in disease contexts. Ongoing research continues to explore its regulatory networks and potential as a biomarker or therapeutic candidate.
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