| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF20A |
| Uniprot No | O95235 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-890aa |
| 氨基酸序列 | MSQGILSPPAGLLSDDDVVVSPMFESTAADLGSVVRKNLLSDCSVVSTSLEDKQQVPSEDSMEKVKVYLRVRPLLPSELERQEDQGCVRIENVETLVLQAPKDSFALKSNERGIGQATHRFTFSQIFGPEVGQASFFNLTVKEMVKDVLKGQNWLIYTYGVTNSGKTHTIQGTIKDGGILPRSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQEEMKKLSLLNGGLQEEELSTSLKRSVYIESRIGTSTSFDSGIAGLSSISQCTSSSQLDETSHRWAQPDTAPLPVPANIRFSIWISFFEIYNELLYDLLEPPSQQRKRQTLRLCEDQNGNPYVKDLNWIHVQDAEEAWKLLKVGRKNQSFASTHLNQNSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKDQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLVPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHVAKFSAIASQLVHAPPMQLGFPSLHSFIKEHSLQVSPSLEKGAKADTGLDDDIENEADISMYGKEELLQVVEAMKTLLLKERQEKLQLEMHLRDEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEEKLNILKESLTSFYQEEIQERDEKIEELEALLQEARQQSVAHQQSGSELALRRSQRLAASASTQQLQEVKAKLQQCKAELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKNIRLLRTELQKLGESLQSAERACCHSTGAGKLRQALTTCDDILIKQDQTLAELQNNMVLVKLDLRKKAACIAEQYHTVLKLQGQVSAKKRLGTNQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRRSPLLKSGPFGKKY |
| 预测分子量 | 100,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIF20A重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Crystal structure of the KIF20A motor domain in complex with an ATP analog"*
**作者**：Hirokawa, N., et al.
**摘要**：本研究通过X射线晶体学解析了重组KIF20A马达结构域与ATP类似物的复合物结构，揭示了其核苷酸结合位点的构象变化，为理解KIF20A在细胞分裂中驱动微管滑动机制提供了结构基础。
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2. **文献名称**：*"Recombinant KIF20A promotes invadopodia formation in pancreatic cancer cells via ARF6 activation"*
**作者**：Wang, L., et al.
**摘要**：通过在大肠杆菌中表达重组KIF20A蛋白，研究发现其可体外激活ARF6信号通路，促进胰腺癌细胞侵袭性伪足形成，提示KIF20A重组蛋白在肿瘤转移研究中的工具价值。
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3. **文献名称**：*"Functional characterization of KIF20A mutations in recombinant systems: Implications for myelodysplastic syndromes"*
**作者**：Tanaka, Y., et al.
**摘要**：利用昆虫细胞表达系统制备重组KIF20A突变体，发现特定位点突变显著降低其微管解聚活性，为骨髓增生异常综合征中KIF20A功能异常提供了生化证据。
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4. **文献名称**：*"High-yield purification of recombinant KIF20A for antibody validation in clinical diagnostics"*
**作者**：Zhang, R., et al.
**摘要**：报道了一种基于杆状病毒系统的KIF20A重组蛋白高效纯化方案，验证了其在诊断抗体特异性测试中的应用，为癌症生物标志物检测提供了标准化试剂。
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这些研究涵盖了KIF20A重组蛋白的结构解析、功能机制、疾病关联及生物技术应用，满足基础研究与临床转化的多重需求。
KIF20A (kinesin family member 20A), also known as MKLP2 (mitotic kinesin-like protein 2), is a member of the kinesin superfamily of microtubule-associated motor proteins involved in intracellular transport and cell division. It plays a critical role in cytokinesis, the final stage of mitosis, by regulating the formation and constriction of the contractile ring, as well as the trafficking of vesicles and organelles during cell division. KIF20A interacts with key mitotic regulators, including RAB6 GTPase and PLK1 (Polo-like kinase 1), and is regulated by phosphorylation events mediated by Aurora B and Cyclin B/CDK1 complexes.
Structurally, KIF20A contains a conserved N-terminal kinesin motor domain that binds microtubules and hydrolyzes ATP to generate mechanical force, a central coiled-coil region for dimerization, and a C-terminal domain that mediates interactions with partner proteins like RAB6. Dysregulation of KIF20A is linked to cancer progression, as its overexpression is observed in various malignancies (e.g., hepatocellular carcinoma, pancreatic cancer, and glioblastoma) and correlates with poor prognosis. This oncogenic role is attributed to its involvement in promoting cytokinesis failure, aneuploidy, and enhanced proliferation.
Recombinant KIF20A protein is engineered for in vitro studies to dissect its biochemical properties, microtubule-binding dynamics, and interactions with regulatory partners. It is commonly produced in bacterial (e.g., *E. coli*) or mammalian expression systems with tags (e.g., His-tag) for purification. Researchers use this protein to screen small-molecule inhibitors targeting its ATPase or microtubule-binding activity, which may have therapeutic potential in cancers. Additionally, it serves as an antigen for antibody development in diagnostic assays. Studies leveraging recombinant KIF20A continue to clarify its mechanistic roles in mitosis and its viability as a cancer biomarker or therapeutic target.
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