| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF18A |
| Uniprot No | Q8NI77 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-898aa |
| 氨基酸序列 | MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTTNQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKRKNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDIKSSLKSNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIERFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKRDHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHHLHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVKIPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTVTLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSKCKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNSSLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR |
| 预测分子量 | 102,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIF18A重组蛋白的3篇文献概览(示例为模拟内容，实际文献需通过学术数据库检索)：
---
1. **文献名称**：*KIF18A Drives Chromosome Congression by Regulating Microtubule Dynamics*
**作者**：Zhu C. et al.
**摘要**：研究通过重组KIF18A蛋白的体外实验，揭示了其通过抑制微管动力学的“刹车”机制促进染色体排列的分子机理，为有丝分裂调控提供新见解。
---
2. **文献名称**：*Structural Insights into KIF18A Motor Domain Conformational Changes*
**作者**：Stumpff J. et al.
**摘要**：利用重组KIF18A蛋白的晶体结构解析，阐明其马达结构域在ATP循环中的构象变化，解释了其选择性结合微管正端的分子基础。
---
3. **文献名称**：*Targeting KIF18A in Cancer Therapy: Biochemical Characterization of Inhibitors*
**作者**：Huang Y. et al.
**摘要**：通过重组KIF18A蛋白的高通量筛选，鉴定出小分子抑制剂，并验证其通过破坏有丝分裂进程抑制肿瘤细胞增殖的潜力。
---
**提示**：建议通过PubMed或Google Scholar搜索关键词“KIF18A recombinant protein”获取最新文献，或关注其功能研究(如纺锤体调控、癌症靶点)相关的论文。
KIF18A, a member of the kinesin superfamily of microtubule-associated motor proteins, plays a critical role in mitotic cell division. As a mitotic kinesin, KIF18A is essential for regulating spindle dynamics and ensuring accurate chromosome alignment during metaphase. It belongs to the kinesin-8 family and exhibits both microtubule plus-end-directed motility and microtubule depolymerase activity. Structurally, KIF18A contains a conserved motor domain that hydrolyzes ATP to generate mechanical force, a coiled-coil stalk for dimerization, and a regulatory tail domain that mediates interactions with other cellular components.
During mitosis, KIF18A localizes to the mitotic spindle, where it suppresses microtubule dynamics by stabilizing kinetochore fibers and limiting microtubule polymerization. This activity promotes proper chromosome congression and prevents errors in chromosome segregation. Dysregulation of KIF18A has been linked to chromosomal instability, mitotic defects, and aneuploidy, which are hallmarks of many cancers. Overexpression of KIF18A is observed in various malignancies, including breast, ovarian, and hepatocellular cancers, making it a potential therapeutic target.
Recombinant KIF18A protein is engineered using expression systems like baculovirus-infected insect cells or mammalian cell cultures to preserve post-translational modifications and functional integrity. Purified recombinant KIF18A serves as a vital tool for in vitro studies, enabling researchers to investigate its enzymatic activity, microtubule-binding properties, and interactions with inhibitors. It also supports high-throughput drug screening campaigns aimed at developing anti-mitotic cancer therapies. Additionally, recombinant KIF18A facilitates structural studies (e.g., cryo-EM) to elucidate mechanisms underlying its role in spindle dynamics and mitotic regulation. Its applications extend to functional assays exploring mitotic checkpoint signaling and chromosome movement mechanics.
×
