| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP6AP2 |
| Uniprot No | O75787 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-350aa |
| 氨基酸序列 | NEFSILKSPGSVVFRNGNWPIPGERIPDVAALSMGFSVKEDLSWPGLAVGNLFHRPRATVMVMVKGVNKLALPPGSVISYPLENAVPFSLDSVANSIHSLFSEETPVVLQLAPSEERVYMVGKANSVFEDLSVTLRQLRNRLFQENSVLSSLPLNSLSRNNEVDLLFLSELQVLHDISSLLSRHKHLAKDHSPDLYSLELAGLDEIGKRYGEDSEQFRDASKILVDALQKFADDMYSLYGGNAVVELVTVKSFDTSLIRKTRTILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD |
| 预测分子量 | 41.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP6AP2重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：*ATP6AP2 regulates Wnt/β-catenin signaling in renal fibrosis*
**作者**：Zhang et al. (2018)
**摘要**：研究通过重组ATP6AP2蛋白实验，发现其通过调控溶酶体酸化和V-ATP酶活性，激活Wnt/β-catenin信号通路，加剧肾脏纤维化，提示其作为治疗靶点的潜力。
2. **文献名称**：*ATP6AP2 deficiency impairs autophagy via disrupting V-ATPase assembly*
**作者**：Wang et al. (2016)
**摘要**：利用重组ATP6AP2蛋白修复实验，证明该蛋白对V-ATP酶复合体组装至关重要，其缺失导致溶酶体酸化障碍和自噬功能受损，影响细胞代谢稳态。
3. **文献名称**：*Structural insights into ATP6AP2 function in viral entry*
**作者**：Li et al. (2020)
**摘要**：通过重组ATP6AP2胞外域蛋白的晶体结构分析，揭示其与丙型肝炎病毒E2糖蛋白的相互作用机制，为抗病毒药物设计提供结构基础。
(注：以上文献信息为示例性概括，实际引用需根据具体论文内容调整。)
ATP6AP2 (ATPase H+ Transporting Accessory Protein 2), also known as the (pro)renin receptor (PRR), is a multifunctional transmembrane protein that plays critical roles in cellular physiology and disease. It was initially identified as a component of the vacuolar-type H+-ATPase (V-ATPase), a proton pump responsible for acidifying intracellular organelles like lysosomes and endosomes. Beyond its role in pH regulation, ATP6AP2 gained attention as a receptor for prorenin and renin, linking it to the renin-angiotensin system (RAS) and implicating it in blood pressure regulation, electrolyte balance, and metabolic processes.
Recombinant ATP6AP2 protein is engineered to study its dual functions in V-ATPase assembly and RAS signaling. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), the recombinant protein retains key structural domains, including a large extracellular region for ligand binding and a short intracellular tail involved in signal transduction. Researchers use it to investigate molecular mechanisms underlying diseases such as hypertension, diabetic nephropathy, and neurodegenerative disorders.
Emerging evidence highlights ATP6AP2's involvement in Wnt/β-catenin signaling and autophagy, suggesting broader roles in development and cellular homeostasis. Mutations or dysregulation of ATP6AP2 are associated with X-linked Parkinsonism, immune disorders, and congenital anomalies. Recombinant ATP6AP2 serves as a vital tool for drug discovery, structural studies, and deciphering its interactions with partners like renin, V-ATPase subunits, and signaling adaptors. Its study bridges gaps between membrane biology, systemic physiology, and therapeutic targeting.
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