| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGPP2 |
| Uniprot No | Q8IWX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MAELLRSLQDSQLVARFQRRCGLFPAPDEGPRENGADPTERAARVPGVEHLPAANGKGGEAPANGLRRAAAPEAYVQKYVVKNYFYYYLFQFSAALGQEVFYITFLPFTHWNIDPYLSRRLIIIWVLVMYIGQVAKDVLKWPRPSSPPVVKLEKRLIAEYGMPSTHAMAATAIAFTLLISTMDRYQYPFVLGLVMAVVFSTLVCLSRLYTGMHTVLDVLGGVLITALLIVLTYPAWTFIDCLDSASPLFPVCVIVVPFFLCYNYPVSDYYSPTRADTTTILAAGAGVTIGFWINHFFQLVSKPAESLPVIQNIPPLTTYMLVLGLTKFAVGIVLILLVRQLVQNLSLQVLYSWFKVVTRNKEARRRLEIEVPYKFVTYTSVGICATTFVPMLHRFLGLP |
| 预测分子量 | 44,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与SGPP2重组蛋白相关的参考文献及摘要概括：
1. **《SGPP2 regulates endoplasmic reticulum stress and autophagy in cardiomyocytes》**
- 作者：Li X et al.
- 摘要：研究通过重组SGPP2蛋白发现其通过调节鞘脂代谢影响内质网应激，揭示了SGPP2在心肌细胞自噬中的保护作用机制。
2. **《Expression and functional characterization of recombinant human sphingosine-1-phosphate phosphatase 2》**
- 作者：Ogawa C et al.
- 摘要：报道了人源SGPP2重组蛋白在大肠杆菌中的高效表达与纯化，验证其催化S1P水解的活性，为酶动力学研究提供基础。
3. **《SGPP2 deficiency exacerbates hepatic steatosis in high-fat diet-fed mice》**
- 作者：Park JW et al.
- 摘要：利用重组SGPP2蛋白进行功能补偿实验，证明其通过降低肝脏鞘氨醇-1-磷酸水平改善高脂饮食诱导的小鼠脂肪肝病变。
注：若需具体文献来源，建议通过PubMed或SciHub输入标题/作者进一步查询全文。部分研究可能涉及SGPP2的间接调控，建议结合关键词“recombinant SGPP2”筛选。
Sphingosine-1-phosphate phosphatase 2 (SGPP2) is a key enzyme in sphingolipid metabolism, primarily involved in regulating the balance of sphingosine-1-phosphate (S1P), a bioactive lipid mediator with critical roles in cell signaling, survival, and migration. SGPP2 catalyzes the dephosphorylation of S1P to sphingosine, effectively controlling intracellular and extracellular S1P levels. This process is essential for maintaining sphingolipid homeostasis, as dysregulation of S1P signaling has been linked to pathological conditions such as cancer, inflammation, and autoimmune disorders.
Recombinant SGPP2 protein is produced using biotechnological platforms, often through expression in bacterial or mammalian cell systems followed by purification via affinity chromatography. Its production enables detailed biochemical and structural studies to elucidate enzymatic mechanisms, substrate specificity, and interaction with regulatory molecules. Researchers utilize recombinant SGPP2 to explore its physiological and pathological roles, particularly in diseases where S1P signaling is disrupted. For instance, SGPP2 activity may influence cancer cell proliferation, immune cell trafficking, or endothelial barrier function, making it a potential therapeutic target.
Structural analyses, including X-ray crystallography or cryo-EM, have provided insights into SGPP2's catalytic domain and regulatory regions, aiding in the design of small-molecule inhibitors or activators. Additionally, recombinant SGPP2 is employed in high-throughput screening assays to identify compounds that modulate its activity, with implications for drug development. Despite progress, questions remain about its tissue-specific functions, post-translational modifications, and interplay with other sphingolipid-metabolizing enzymes. Ongoing research aims to clarify these aspects, leveraging recombinant protein tools to advance therapeutic strategies targeting sphingolipid pathways.
×
