| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | coaA |
| Uniprot No | Q8TE04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 231-598aa |
| 氨基酸序列 | KNRPPFPWFGMDIGGTLVKLVYFEPKDITAEEEQEEVENLKSIRKYLTSNTAYGKTGIRDVHLELKNLTMCGRKGNLHFIRFPSCAMHRFIQMGSEKNFSSLHTTLCATGGGAFKFEEDFRMIADLQLHKLDELDCLIQGLLYVDSVGFNGKPECYYFENPTNPELCQKKPYCLDNPYPMLLVNMGSGVSILAVYSKDNYKRVTGTSLGGGTFLGLCCLLTGCETFEEALEMAAKGDSTNVDKLVKDIYGGDYERFGLQGSAVASSFGNMMSKEKRDSISKEDLARATLVTITNNIGSIARMCALNENIDRVVFVGNFLRINMVSMKLLAYAMDFWSKGQLKALFLEHEGYFGAVGALLELFKMTDDK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **coaA重组蛋白** 的3篇参考文献概览(注：文献为模拟示例，实际需根据数据库检索调整)：
---
1. **文献名称**：*Heterologous expression and functional characterization of CoaA from Staphylococcus aureus in E. coli*
**作者**：Wang Y, et al.
**摘要**：研究克隆了金黄色葡萄球菌的coaA基因，在大肠杆菌中成功表达并纯化重组蛋白，证实其磷酸泛酰巯基乙胺基转移酶活性，为开发抗菌靶点提供依据。
2. **文献名称**：*Crystal structure and biochemical analysis of CoaA in coenzyme A biosynthesis*
**作者**：Li X, Zhang R.
**摘要**：解析了重组CoaA蛋白的晶体结构，结合酶动力学实验阐明其催化机制，发现底物结合位点的关键残基突变显著降低酶活性。
3. **文献名称**：*Optimization of CoaA recombinant protein production using a high-cell-density fermentation system*
**作者**：Chen H, et al.
**摘要**：通过优化诱导条件和发酵策略，显著提高了CoaA重组蛋白在大肠杆菌中的产量，为其工业化应用(如辅酶A生产)奠定基础。
---
**提示**：实际文献建议通过 **PubMed/Google Scholar** 检索关键词 *"coaA recombinant protein"、"coaA cloning expression"* 或结合具体物种(如*Staphylococcus*)，并筛选近年研究。
**Background of CoaA Recombinant Protein**
CoaA, encoded by the *coaA* gene, is a critical enzyme in the biosynthesis of coenzyme A (CoA), a universal cofactor essential for numerous metabolic pathways, including fatty acid metabolism, citric acid cycle, and cellular detoxification processes. Functioning as a pantothenate kinase (PanK), CoaA catalyzes the ATP-dependent phosphorylation of pantothenate (vitamin B5), the first and rate-limiting step in CoA synthesis. This reaction converts pantothenate into 4'-phosphopantothenate, initiating a multi-enzyme pathway to produce CoA.
In prokaryotes, CoaA is vital for bacterial survival, making it a potential target for antimicrobial drug development. Its role in central metabolism and absence in humans enhance its appeal as a therapeutic target. Recombinant CoaA protein is typically produced using heterologous expression systems, such as *E. coli*, where the *coaA* gene is cloned into expression vectors under inducible promoters (e.g., T7 or lac). The protein is then purified via affinity chromatography (e.g., His-tag) for functional studies.
Research on recombinant CoaA focuses on elucidating its structure, enzymatic mechanisms, and regulatory interactions. Structural studies using X-ray crystallography or cryo-EM provide insights into substrate binding and catalysis, aiding in inhibitor design. Additionally, CoaA activity is tightly regulated by feedback inhibition via CoA or its thioesters, a feature critical for maintaining metabolic balance. Dysregulation of CoA synthesis is linked to metabolic disorders and pathogen virulence, underscoring its biomedical relevance.
Applications of recombinant CoaA include high-throughput screening for antimicrobial compounds and metabolic engineering to optimize CoA-dependent pathways in industrial microorganisms. Its study also contributes to understanding eukaryotic PanK isoforms, which are implicated in neurodegenerative diseases like PKAN (Pantothenate Kinase-Associated Neurodegeneration). Overall, CoaA recombinant protein serves as a key tool in both basic research and biotechnological innovation.
×
