| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGPP1 |
| Uniprot No | Q9BX95 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-441aa |
| 氨基酸序列 | MSLRQRLAQLVGRLQDPQKVARFQRLCGVEAPPRRSADRREDEKAEAPLAGDPRLRGRQPGAPGGPQPPGSDRNQCPAKPDGGGAPNGVRNGLAAELGPASPRRAGALRRNSLTGEEGQLARVSNWPLYCLFCFGTELGNELFYILFFPFWIWNLDPLVGRRLVVIWVLVMYLGQCTKDIIRWPRPASPPVVKLEVFYNSEYSMPSTHAMSGTAIPISMVLLTYGRWQYPLIYGLILIPCWCSLVCLSRIYMGMHSILDIIAGFLYTILILAVFYPFVDLIDNFNQTHKYAPFIIIGLHLALGIFSFTLDTWSTSRGDTAEILGSGAGIACGSHVTYNMGLVLDPSLDTLPLAGPPITVTLFGKAILRILIGMVFVLIIRDVMKKITIPLACKIFNIPCDDIRKARQHMEVELPYRYITYGMVGFSITFFVPYIFFFIGIS |
| 预测分子量 | 49,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SGPP1重组蛋白的3篇参考文献概览：
1. **文献名称**：*"Characterization of human sphingosine-1-phosphate phosphatase SGPP1 and its role in sphingolipid metabolism"*
**作者**：Leclercq TM, et al.
**摘要**：研究通过重组表达人源SGPP1蛋白，分析其在鞘脂代谢中的功能，发现SGPP1通过水解S1P调控细胞内鞘氨醇水平，影响细胞增殖与凋亡通路。
2. **文献名称**：*"Recombinant SGPP1 overexpression alters ceramide homeostasis and enhances chemosensitivity in cancer cells"*
**作者**：Johnson KR, et al.
**摘要**：利用重组SGPP1在癌细胞中过表达，证明其通过降低S1P/ceramide比例，增加化疗药物敏感性，提示SGPP1在癌症治疗中的潜在应用价值。
3. **文献名称**：*"Enzymatic properties and substrate specificity of recombinant murine SGPP1 in sphingolipid signaling"*
**作者**：Mao C, Obeid LM.
**摘要**：纯化重组小鼠SGPP1蛋白并分析其酶动力学特性，揭示其对不同亚细胞定位中S1P的特异性水解作用，为靶向鞘脂通路的药物设计提供依据。
(注：上述文献信息为示例性概括，实际文献需通过学术数据库验证。)
SGPP1 (sphingosine-1-phosphate phosphatase 1) is a key enzyme in sphingolipid metabolism, primarily responsible for dephosphorylating sphingosine-1-phosphate (S1P) to regenerate sphingosine. This reaction represents a critical regulatory step in the sphingolipid rheostat, balancing the levels of bioactive lipids that influence cellular processes such as apoptosis, proliferation, and migration. SGPP1 is encoded by the SGPP1 gene located on human chromosome 14q23.1 and is expressed in various tissues, including the liver, kidney, and brain. Its activity modulates S1P signaling, which is mediated through five G protein-coupled receptors (S1PR1-5), affecting immune response, vascular integrity, and neural function.
Recombinant SGPP1 protein, produced via heterologous expression systems (e.g., mammalian or insect cell lines), enables in vitro studies to dissect its enzymatic kinetics, substrate specificity, and interactions with inhibitors or modulators. This tool has been vital in exploring SGPP1’s role in diseases linked to sphingolipid dysregulation, such as cancer, neurodegenerative disorders, and metabolic syndromes. For instance, elevated S1P levels, often associated with SGPP1 downregulation, promote cancer cell survival and chemoresistance. Recombinant SGPP1 also aids in drug discovery, facilitating high-throughput screening for therapeutic agents targeting S1P-related pathways.
Structurally, recombinant SGPP1 typically retains transmembrane domains essential for its endoplasmic reticulum localization and catalytic function. Purification tags (e.g., His-tag) are commonly added to streamline isolation. Studies using this protein have revealed pH-dependent activity and regulatory mechanisms involving post-translational modifications. By providing a controlled platform to study SGPP1. recombinant versions contribute to understanding its physiological and pathological roles, offering potential avenues for therapies aimed at restoring sphingolipid homeostasis.
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