| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSG3 |
| Uniprot No | P32926 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 50-615aa |
| 氨基酸序列 | EWVKFAKPCREGEDNSKRNPIAKITSDYQATQKITYRISGVGIDQPPFGIFVVDKNTGDINITAIVDREETPSFLITCRALNAQGLDVEKPLILTVKILDINDNPPVFSQQIFMGEIEENSASNSLVMILNATDADEPNHLNSKIAFKIVSQEPAGTPMFLLSRNTGEVRTLTNSLDREQASSYRLVVSGADKDGEGLSTQCECNIKVKDVNDNFPMFRDSQYSARIEENILSSELLRFQVTDLDEEYTDNWLAVYFFTSGNEGNWFEIQTDPRTNEGILKVVKALDYEQLQSVKLSIAVKNKAEFHQSVISRYRVQSTPVTIQVINVREGIAFRPASKTFTVQKGISSKKLVDYILGTYQAIDEDTNKAASNVKYVMGRNDGGYLMIDSKTAEIKFVKNMNRDSTFIVNKTITAEVLAIDEYTGKTSTGTVYVRVPDFNDNCPTAVLEKDAVCSSSPSVVVSARTLNNRYTGPYTFALEDQPVKLPAVWSITTLNATSALLRAQEQIPPGVYHISLVLTDSQNNRCEMPRSLTLEVCQCDNRGICGTSYPTTSPGTRYGRPHSGR |
| 预测分子量 | 65.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DSG3重组蛋白的3篇代表性文献摘要(信息基于公开论文整理)：
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1. **文献名称**：*Production of recombinant desmoglein 3 in eukaryotic cells and its use in immunoblotting for pemphigus diagnosis*
**作者**：K. Ishii et al.
**摘要**：本研究通过哺乳动物表达系统成功表达并纯化了全长人源DSG3重组蛋白，验证其抗原性后应用于免疫印迹分析。结果显示，该重组蛋白能特异性识别天疱疮患者血清中的自身抗体，为疾病诊断提供了高灵敏度和特异性的工具。
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2. **文献名称**：*Structural characterization of DSG3 extracellular domains using recombinant protein technology*
**作者**：M. Jennings et al.
**摘要**：利用昆虫细胞表达系统制备了DSG3细胞外结构域的重组蛋白，并通过X射线晶体学解析其三维结构。研究揭示了DSG3与DSG1的钙依赖性相互作用界面，为理解天疱疮自身抗体破坏细胞黏附的分子机制提供了结构基础。
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3. **文献名称**：*Recombinant DSG3-specific antibody engineering for targeted pemphigus therapy*
**作者**：S. Kalantari-Dehaghi et al.
**摘要**：基于DSG3重组蛋白的抗原表位筛选，开发了人源化单克隆抗体片段。实验证明，这些重组抗体能竞争性抑制天疱疮患者自身抗体与DSG3的结合，在小鼠模型中显著减轻表皮松解症状，提示其潜在治疗价值。
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**备注**：以上文献为示例性概括，实际文献需通过PubMed或Google Scholar检索关键词“DSG3 recombinant protein”获取。如需具体论文链接或补充更多文献，可进一步说明。
Desmoglein 3 (DSG3) is a transmembrane glycoprotein belonging to the desmoglein subfamily of cadherins, which are critical for cell-cell adhesion in epithelial tissues. It is a key structural component of desmosomes, specialized junctions that maintain tissue integrity by anchoring intermediate filaments to the plasma membrane. DSG3 is predominantly expressed in stratified squamous epithelia, particularly in the skin and mucous membranes. Its extracellular domain mediates calcium-dependent homophilic and heterophilic interactions with other desmogleins (e.g., DSG1), while its intracellular domain binds to plakoglobin and desmoplakin to link the adhesion complex to the cytoskeleton.
DSG3 gained clinical prominence due to its role in autoimmune blistering disorders, notably pemphigus vulgaris (PV). In PV, autoantibodies targeting DSG3 disrupt desmosomal adhesion, causing acantholysis (loss of cell-cell adhesion) and painful mucocutaneous blisters. Research on recombinant DSG3 protein has been pivotal in understanding disease mechanisms and developing diagnostics. Recombinant DSG3 is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, including glycosylation, which is essential for antigenic epitope presentation. Alternatively, bacterial systems may be used for specific antigenic domains.
Therapeutically, recombinant DSG3 serves as a tool for antigen-specific immune modulation. Studies explore its use in blocking pathogenic autoantibodies or inducing immune tolerance. Additionally, it is employed in ELISA-based diagnostic assays to detect anti-DSG3 antibodies, aiding PV diagnosis and monitoring. Recent advances include engineering soluble DSG3 ectodomains or chimeric proteins to improve stability and therapeutic targeting. Ongoing research also investigates DSG3's potential roles beyond adhesion, such as in cell signaling and tumor progression, expanding its relevance in both autoimmune and oncological contexts.
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