| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHN1 |
| Uniprot No | P15882 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-459aa |
| 氨基酸序列 | ALTLFDTDE YRPPVWKSYL YQLQQEAPHP RRITCTCEVE NRPKYYGREF HGMISREAAD QLLIVAEGSY LIRESQRQPG TYTLALRFGS QTRNFRLYYD GKHFVGEKRF ESIHDLVTDG LITLYIETKA AEYIAKMTIN PIYEHVGYTT LNREPAYKKH MPVLKETHDE RDSTGQDGVS EKRLTSLVRR ATLKENEQIP KYEKIHNFKV HTFRGPHWCE YCANFMWGLI AQGVKCADCG LNVHKQCSKM VPNDCKPDLK HVKKVYSCDL TTLVKAHTTK RPMVVDMCIR EIESRGLNSE GLYRVSGFSD LIEDVKMAFD RDGEKADISV NMYEDINIIT GALKLYFRDL PIPLITYDAY PKFIESAKIM DPDEQLETLH EALKLLPPAH CETLRYLMAH LKRVTLHEKE NLMNAENLGI VFGPTLMRSP ELDAMAALND IRYQRLVVEL LIKNEDILF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHN1重组蛋白的3篇参考文献示例(注：内容为虚构，仅作格式参考)：
1. **"Expression and Purification of Recombinant CHN1 Protein in E. coli"**
*作者：Zhang L., et al.*
摘要：本研究成功构建了CHN1重组蛋白的原核表达系统，优化了纯化条件，并验证了其与Rac1 GTP酶的相互作用活性。
2. **"CHN1 Recombinant Protein Regulates Neuronal Growth Cone Collapse"**
*作者：Smith J.R., Brown K.T.*
摘要：通过体外实验证明，重组CHN1蛋白通过抑制Rac1信号通路，调控神经元生长锥的导向与突触可塑性。
3. **"Structural Analysis of α2-Chimaerin (CHN1) Using Cryo-EM"**
*作者：Wang Y., et al.*
摘要：利用冷冻电镜解析了重组CHN1蛋白的三维结构，揭示了其SH2结构域与磷酸化酪氨酸结合的关键位点。
如需真实文献，建议通过PubMed或Sci-Hub检索关键词“CHN1 recombinant protein”获取。
CHN1. also known as chimerin 1. is a GTPase-activating protein (GAP) encoded by the CHN1 gene in humans. It belongs to the chimerin family, which regulates intracellular signaling by inactivating Rac and Cdc42. small GTPases involved in cytoskeletal dynamics, cell adhesion, and neuronal development. CHN1 contains a C-terminal GAP domain and an N-terminal SH2 domain, enabling interactions with phosphorylated tyrosine kinase receptors. Initially identified in the brain, it is highly expressed in the nervous system, where it modulates axon guidance, synaptic plasticity, and neurite outgrowth by controlling Rho GTPase activity.
Research highlights its role in neurodevelopmental processes and neurodegenerative diseases. Mutations in CHN1 are linked to Duane retraction syndrome, a congenital eye movement disorder, and have been implicated in psychiatric conditions like schizophrenia. Beyond neurology, CHN1 dysregulation is observed in cancers, including breast and prostate cancer, where it influences cell migration and metastasis. Its tumor-suppressive or oncogenic effects appear context-dependent, varying with cellular environment and signaling pathways.
Recombinant CHN1 protein, produced via bacterial or mammalian expression systems, is widely used to study GTPase interactions, signal transduction mechanisms, and drug targeting. Purified CHN1 serves as a tool for in vitro assays, structural studies, and screening therapeutic compounds aimed at modulating Rho GTPase pathways. Its applications extend to investigating neural repair strategies and cancer therapeutics, emphasizing its dual significance in basic research and translational medicine. Ongoing studies continue to explore its regulatory networks and potential as a biomarker or therapeutic target.
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