| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGM4 |
| Uniprot No | P49221 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-684aa |
| 氨基酸序列 | MMDASKELQVLHIDFLNQDNAVSHHTWEFQTSSPVFRRGQVFHLRLVLNQPLQSYHQLKLEFSTGPNPSIAKHTLVVLDPRTPSDHYNWQATLQNESGKEVTVAVTSSPNAILGKYQLNVKTGNHILKSEENILYLLFNPWCKEDMVFMPDEDERKEYILNDTGCHYVGAARSIKCKPWNFGQFEKNVLDCCISLLTESSLKPTDRRDPVLVCRAMCAMMSFEKGQGVLIGNWTGDYEGGTAPYKWTGSAPILQQYYNTKQAVCFGQCWVFAGILTTVLRALGIPARSVTGFDSAHDTERNLTVDTYVNENGEKITSMTHDSVWNFHVWTDAWMKRPDLPKGYDGWQAVDATPQERSQGVFCCGPSPLTAIRKGDIFIVYDTRFVFSEVNGDRLIWLVKMVNGQEELHVISMETTSIGKNISTKAVGQDRRRDITYEYKYPEGSSEERQVMDHAFLLLSSEREHRRPVKENFLHMSVQSDDVLLGNSVNFTVILKRKTAALQNVNILGSFELQLYTGKKMAKLCDLNKTSQIQGQVSEVTLTLDSKTYINSLAILDDEPVIRGFIIAEIVESKEIMASEVFTSFQYPEFSIELPNTGRIGQLLVCNCIFKNTLAIPLTDVKFSLESLGISSLQTSDHGTVQPGETIQSQIKCTPIKTGPKKFIVKLSSKQVKEINAQKIVLITK |
| 预测分子量 | 90.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGM4重组蛋白的3篇参考文献示例(注：文献信息为模拟示例，实际引用需核实真实来源)：
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1. **文献名称**: *Recombinant human TGM4 induces extracellular matrix remodeling in prostate cancer cells*
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌系统表达重组人TGM4蛋白，发现其在前列腺癌细胞中激活基质金属蛋白酶(MMPs)，促进细胞外基质降解，可能与肿瘤转移相关。
2. **文献名称**: *Expression and functional characterization of TGM4 in sperm capacitation*
**作者**: Zhang Y, et al.
**摘要**: 利用昆虫细胞系统重组表达TGM4蛋白，证实其在精子顶体反应中催化蛋白质交联，影响精子穿透卵子透明带的能力。
3. **文献名称**: *TGM4 recombinant protein as a potential biomarker in chronic kidney disease*
**作者**: Brown K, et al.
**摘要**: 通过哺乳动物细胞表达重组TGM4.发现其在肾脏纤维化模型中异常升高，可能作为疾病进展的生物标志物。
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**说明**：
- TGM4(Transglutaminase 4)研究多聚焦于生殖系统(如精子功能)和癌症(如前列腺癌)，重组蛋白常用于机制研究或应用开发。
- 实际文献需通过PubMed/Google Scholar搜索关键词“TGM4 recombinant”“transglutaminase 4 expression”获取。
- 实验系统(如大肠杆菌、哺乳动物细胞)和功能分析(酶活性、疾病模型)是此类研究的常见方向。
建议结合具体研究领域进一步筛选文献。
**Background of TGM4 Recombinant Protein**
Transglutaminase 4 (TGM4), a member of the transglutaminase enzyme family, plays a critical role in post-translational protein modification by catalyzing calcium-dependent cross-linking reactions. Primarily expressed in the prostate gland, TGM4 is implicated in reproductive biology, including semen coagulation and sperm motility regulation. Its structural domains, such as the catalytic core and calcium-binding regions, enable substrate recognition and enzymatic activity, targeting proteins like semenogelins in seminal fluid.
Recombinant TGM4 protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian cell systems) to express the purified enzyme for research and therapeutic applications. The recombinant form retains native enzymatic properties, allowing studies on its physiological roles and pathological associations. For instance, aberrant TGM4 expression has been linked to prostate cancer progression, where it may influence tumor microenvironment dynamics or cell survival pathways. Additionally, TGM4 polymorphisms or dysregulation are under investigation for potential connections to male infertility.
The development of recombinant TGM4 has advanced diagnostic and drug discovery efforts. It serves as a tool for screening inhibitors or modulators in cancer therapy and aids in elucidating molecular mechanisms underlying prostate-related disorders. Furthermore, recombinant TGM4 facilitates antibody production for immunohistochemical detection in clinical samples, enhancing disease biomarker research.
In summary, TGM4 recombinant protein bridges basic science and translational applications, offering insights into reproductive health, oncology, and protein biochemistry, while supporting innovations in therapeutic targeting and diagnostic development.
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