| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTGIS |
| Uniprot No | Q16647 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-500aa |
| 氨基酸序列 | MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP |
| 预测分子量 | 57,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟示例的3篇关于PTGIS重组蛋白的参考文献(注：文献信息为示例性构建，非真实存在)：
---
1. **标题**：Recombinant Expression and Functional Characterization of Human Prostacyclin Synthase in E. coli
**作者**：Yamamoto A, et al.
**摘要**：本研究成功在大肠杆菌系统中重组表达了具有酶活性的人源PTGIS蛋白，并通过亲和层析纯化。实验证实重组PTGIS可将前列腺素H2转化为前列环素(PGI2)，为体外酶活研究提供了可靠工具。
2. **标题**：Crystal Structure Analysis of PTGIS Recombinant Protein Reveals Substrate Binding Sites
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了重组PTGIS蛋白的三维结构，揭示了其与底物前列腺素H2的结合区域及关键氨基酸残基，为设计靶向PTGIS的药物提供了结构基础。
3. **标题**：Role of Recombinant PTGIS in Vascular Inflammation Models
**作者**：Smith J, et al.
**摘要**：利用哺乳动物细胞表达的重组PTGIS蛋白，研究发现其过表达可显著抑制内皮细胞炎症因子释放，提示其在动脉粥样硬化治疗中的潜在应用价值。
---
提示：实际研究中建议通过PubMed或Google Scholar以关键词“PTGIS recombinant protein”检索最新文献，并优先选择高影响力期刊论文。
PTGIS (Prostaglandin I2 Synthase), also known as prostacyclin synthase, is a member of the cytochrome P450 superfamily (CYP8A1) that catalyzes the conversion of prostaglandin H2 (PGH2) to prostacyclin (PGI2), a potent vasodilator and inhibitor of platelet aggregation. This enzyme plays a critical role in cardiovascular homeostasis, blood pressure regulation, and inflammatory responses. Dysregulation of PTGIS activity or expression has been linked to vascular diseases, including atherosclerosis, hypertension, and pulmonary arterial hypertension.
Recombinant PTGIS protein is engineered using genetic cloning techniques to express the enzyme in heterologous systems such as *E. coli*, insect cells, or mammalian cell lines. This approach allows large-scale production of functional PTGIS for research and therapeutic applications. The recombinant protein retains catalytic activity, enabling studies on its structure-function relationships, substrate specificity, and interactions with inhibitors or modulators. Researchers often employ affinity tags (e.g., His-tag) to facilitate purification via chromatography.
In biomedical research, recombinant PTGIS is used to investigate the molecular mechanisms underlying cardiovascular pathologies and to screen potential drugs targeting the prostacyclin pathway. It also serves as a tool to study genetic mutations affecting enzyme activity and their correlation with disease phenotypes. Additionally, recombinant PTGIS has potential therapeutic applications, such as enzyme replacement strategies or as a component in bioengineered vascular grafts to improve patency. Its role in modulating oxidative stress and endothelial function further underscores its relevance in metabolic and inflammatory disorders.
×
