| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EP1 |
| Uniprot No | P34995 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402aa |
| 氨基酸序列 | MSPCGPLNLSLAGEATTCAAPWVPNTSAVPPSGASPALPIFSMTLGAVSNLLALALLAQAAGRLRRRRSAATFLLFVASLLATDLAGHVIPGALVLRLYTAGRAPAGGACHFLGGCMVFFGLCPLLLGCGMAVERCVGVTRPLLHAARVSVARARLALAAVAAVALAVALLPLARVGRYELQYPGTWCFIGLGPPGGWRQALLAGLFASLGLVALLAALVCNTLSGLALLRARWRRRSRRPPPASGPDSRRRWGAHGPRSASASSASSIASASTFFGGSRSSGSARRARAHDVEMVGQLVGIMVVSCICWSPMLVLVALAVGGWSSTSLQRPLFLAVRLASWNQILDPWVYILLRQAVLRQLLRLLPPRAGAKGGPAGLGLTPSAWEASSLRSSRHSGLSHF |
| 预测分子量 | 41,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与EP1重组蛋白相关的参考文献示例(注：部分文献信息为模拟概括，实际引用请核实原文)：
1. **《Functional characterization of recombinant human EP1 receptor in inflammatory pain signaling》**
- 作者：Smith A, et al.
- 摘要：研究通过大肠杆菌表达系统制备重组人EP1受体蛋白，验证其与前列腺素E2(PGE2)的结合活性，并发现EP1受体介导的钙离子信号通路在炎症性疼痛中的关键作用。
2. **《High-yield expression and purification of EP1 receptor for structural studies》**
- 作者：Li X, Wang Y.
- 摘要：报道了一种昆虫细胞-杆状病毒系统高效表达EP1重组蛋白的方法，结合亲和层析技术纯化获得高纯度蛋白，为后续冷冻电镜解析EP1受体三维结构奠定基础。
3. **《EP1 receptor antagonists: Screening platform development using recombinant protein》**
- 作者：Garcia R, et al.
- 摘要：利用哺乳动物细胞表达的重组EP1蛋白建立药物筛选模型，鉴定出新型小分子拮抗剂，可抑制EP1介导的细胞增殖，为癌症靶向治疗提供候选化合物。
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**说明**：
- EP1通常指前列腺素E受体亚型之一，研究多聚焦于其在炎症、疼痛和肿瘤中的功能。
- 实际文献需通过PubMed、Web of Science等平台以关键词“EP1 receptor recombinant”或“EP1 protein expression”检索，并选择领域内权威期刊(如*JBC*、*Nature Structural Biology*等)。
**Background of EP1 Recombinant Protein**
The EP1 recombinant protein is a genetically engineered protein derived from the EP1 antigen, a critical component often associated with pathogenic organisms or cellular signaling pathways. Originally identified in studies involving viral or bacterial pathogens, EP1 is known for its role in mediating host-pathogen interactions, such as facilitating cellular entry, immune evasion, or modulating host cell functions. For instance, in certain viral contexts, EP1 may correspond to an envelope or surface protein essential for viral attachment and infection.
Recombinant EP1 is produced using advanced biotechnological methods, typically involving the insertion of the EP1 gene into expression vectors (e.g., plasmids), which are then transfected into host systems like *E. coli*, yeast, or mammalian cells. This approach enables scalable production of the protein with high purity and consistency. The recombinant form retains the structural and functional properties of the native protein, making it valuable for research and therapeutic applications.
In biomedical research, EP1 recombinant protein serves as a key tool for studying immune responses, developing diagnostic assays (e.g., ELISA, lateral flow tests), and screening potential therapeutics, such as monoclonal antibodies or inhibitors targeting EP1-mediated pathways. In vaccine development, it may act as an antigen to elicit protective immunity. Additionally, its role in cellular signaling or disease mechanisms (e.g., cancer, infectious diseases) has spurred interest in exploring EP1 as a biomarker or therapeutic target.
The production and application of EP1 recombinant protein highlight its versatility in bridging basic science and clinical innovation, offering insights into disease mechanisms and accelerating the development of targeted interventions.
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