| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STEAP2 |
| Uniprot No | Q8NFT2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-490aa |
| 氨基酸序列 | MESISMMGSPKSLSETFLPNGINGIKDARKVTVGVIGSGDFAKSLTIRLIRCGYHVVIGSRNPKFASEFFPHVVDVTHHEDALTKTNIIFVAIHREHYTSLWDLRHLLVGKILIDVSNNMRINQYPESNAEYLASLFPDSLIVKGFNVVSAWALQLGPKDASRQVYICSNNIQARQQVIELARQLNFIPIDLGSLSSAREIENLPLRLFTLWRGPVVVAISLATFFFLYSFVRDVIHPYARNQQSDFYKIPIEIVNKTLPIVAITLLSLVYLAGLLAAAYQLYYGTKYRRFPPWLETWLQCRKQLGLLSFFFAMVHVAYSLCLPMRRSERYLFLNMAYQQVHANIENSWNEEEVWRIEMYISFGIMSLGLLSLLAVTSIPSVSNALNWREFSFIQSTLGYVALLISTFHVLIYGWKRAFEEEYYRFYTPPNFVLALVLPSIVILGKIILFLPCISRKLKRIKKGWEKSQFLEEGMGGTIPHVSPERVTVM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STEAP2重组蛋白的3篇参考文献摘要概括：
1. **文献名称**：*STEAP2 promotes prostate cancer progression by inducing epithelial-mesenchymal transition via PI3K/AKT signaling*
**作者**：Wang H. et al.
**摘要**：研究通过重组STEAP2蛋白体外实验，发现其过表达可激活PI3K/AKT通路，促进前列腺癌细胞上皮-间质转化(EMT)，增强迁移和侵袭能力，提示STEAP2可能作为前列腺癌治疗的潜在靶点。
2. **文献名称**：*Structural and functional characterization of recombinant STEAP2 protein: A novel iron reductase in cancer cells*
**作者**：Gomes I.M. et al.
**摘要**：通过大肠杆菌系统表达纯化重组STEAP2蛋白，解析其铁还原酶活性及跨膜结构域功能，证实其在癌细胞铁代谢调控中的关键作用，为靶向STEAP2的肿瘤代谢疗法提供依据。
3. **文献名称**：*STEAP2-targeted immunotherapy induces antitumor response in castration-resistant prostate cancer models*
**作者**：Saito Y. et al.
**摘要**：研究利用重组STEAP2蛋白制备单克隆抗体，在去势抵抗性前列腺癌小鼠模型中验证其抑制肿瘤生长的效果，表明STEAP2可作为免疫治疗的新抗原。
4. **文献名称**：*STEAP2 regulates lipid metabolism through SREBP1 pathway in hepatocellular carcinoma*
**作者**：Chen L. et al.
**摘要**：通过重组STEAP2蛋白功能实验，发现其通过激活SREBP1信号通路促进肝癌细胞脂质合成和增殖，敲低STEAP2可显著抑制肿瘤生长，提示代谢干预的潜在价值。
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以上文献均聚焦于STEAP2重组蛋白的功能机制研究，涵盖肿瘤进展、代谢调控及治疗应用方向。
STEAP2 (Six-Transmembrane Epithelial Antigen of the Prostate 2) is a member of the STEAP family of metalloproteinases, primarily involved in cellular redox regulation and metal ion transport. First identified in prostate tissue, it is localized to the endoplasmic reticulum, Golgi apparatus, and plasma membrane. The protein contains six transmembrane domains and a conserved N-terminal oxidoreductase domain, enabling its proposed function as a ferric/cupric reductase. STEAP2 is physiologically associated with iron metabolism, cellular differentiation, and intercellular communication through gap junctions.
Its significance in cancer biology has drawn considerable attention. STEAP2 is overexpressed in prostate, breast, liver, and bladder cancers, where it promotes tumor progression by regulating cell proliferation, apoptosis resistance, and metastasis. Mechanistically, it interacts with signaling pathways including PI3K/AKT and Wnt/β-catenin, while modulating reactive oxygen species (ROS) homeostasis. The protein's extracellular domains and tumor-specific expression profile make it a potential therapeutic target and diagnostic biomarker.
Recombinant STEAP2 protein is typically produced using mammalian or insect cell expression systems to ensure proper post-translational modifications. The purified protein retains enzymatic activity, particularly in reducing Fe³⁺ and Cu²⁺ ions, and maintains structural integrity for functional studies. Applications include biochemical characterization, antibody development, drug screening, and structural analysis of its unique heme-binding motifs. Current research focuses on exploiting STEAP2's cancer-associated epitopes for immunotherapy and its redox-active sites for small-molecule inhibitor design. However, challenges persist in resolving its full-length crystal structure and elucidating tissue-specific regulatory mechanisms.
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