| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GCNF |
| Uniprot No | Q15406 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-480aa |
| 氨基酸序列 | MERDEPPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTISV SDDRAEQRTC LICGDRATGL HYGIISCEGC KGFFKRSICN KRVYRCSRDK NCVMSRKQRN RCQYCRLLKC LQMGMNRKAI REDGMPGGRN KSIGPVQISE EEIERIMSGQ EFEEEANHWS NHGDSDHSSP GNRASESNQP SPGSTLSSSR SVELNGFMAF REQYMGMSVP PHYQYIPHLF SYSGHSPLLP QQARSLDPQS YSLIHQLLSA EDLEPLGTPM LIEDGYAVTQ AELFALLCRL ADELLFRQIA WIKKLPFFCE LSIKDYTCLL SSTWQELILL SSLTVYSKQI FGELADVTAK YSPSDEELHR FSDEGMEVIE RLIYLYHKFH QLKVSNEEYA CMKAINFLNQ DIRGLTSASQ LEQLNKRYWY ICQDFTEYKY THQPNRFPDL MMCLPEIRYI AGKMVNVPLE QLPLLFKVVL HSCKTSVGKE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GCNF(Germ Cell Nuclear Factor)重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: "The orphan nuclear receptor GCNF recruits DNA methyltransferase for transcriptional repression in mouse embryonic stem cells"
**作者**: Gu P, et al.
**摘要**: 研究揭示了重组GCNF蛋白在小鼠胚胎干细胞中通过招募DNA甲基转移酶抑制靶基因转录的分子机制，表明GCNF在表观遗传调控和干细胞分化中起关键作用。
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2. **文献名称**: "Structural and functional analysis of germ cell nuclear factor (GCNF) as a transcriptional repressor"
**作者**: Cooney AJ, et al.
**摘要**: 通过重组GCNF蛋白的体外表达与结构分析，阐明了其DNA结合域和配体结合域的结构特征，并证明GCNF通过结合特定DNA序列抑制生殖细胞分化相关基因的表达。
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3. **文献名称**: "GCNF-dependent regulation of pluripotency and differentiation in early embryos"
**作者**: Susens U, et al.
**摘要**: 利用重组GCNF蛋白进行功能实验，发现其在早期胚胎发育中通过调控Oct4等多能性因子的表达，参与胚胎干细胞多能性维持与向生殖细胞谱系分化的转换。
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(注：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。)
**Background of GCNF Recombinant Protein**
GCNF (Germ Cell Nuclear Factor), also known as NR6A1. is a member of the nuclear receptor superfamily, which plays critical roles in transcriptional regulation. Initially identified for its expression in germ cells, GCNF is a ligand-binding transcription factor characterized by a conserved DNA-binding domain (DBD) and a ligand-binding domain (LBD). Unlike many nuclear receptors, GCNF lacks a classical activation function and primarily acts as a transcriptional repressor. It regulates gene expression by binding to specific DNA response elements, often repressing target genes involved in embryonic development, germ cell differentiation, and metabolic processes.
GCNF is essential during early embryogenesis, particularly in regulating the transition from pluripotency to differentiation in embryonic stem cells. Studies in knockout mice have highlighted its role in neural development, organogenesis, and germ cell maturation, with GCNF deficiency leading to embryonic lethality or severe developmental defects. Its involvement in silencing pluripotency genes, such as *Oct4* and *Nanog*, underscores its importance in maintaining developmental timelines.
Recombinant GCNF protein is produced using expression systems like *E. coli* or mammalian cells, enabling biochemical and functional studies. Purified GCNF is utilized to investigate its DNA-binding properties, interaction with co-repressors, and regulatory mechanisms in vitro. As an orphan receptor (endogenous ligand unknown), structural studies of recombinant GCNF help elucidate its ligand-binding pocket and conformational dynamics, offering insights into potential therapeutic targeting.
Research on GCNF recombinant protein also extends to disease models, including cancer and neurodegenerative disorders, where dysregulation of GCNF-mediated pathways is implicated. Its role in metabolic regulation further links it to conditions like diabetes and obesity. Overall, GCNF remains a pivotal molecule for understanding developmental biology and designing interventions for related pathologies.
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