| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Grb7 |
| Uniprot No | Q14451 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-532aa |
| 氨基酸序列 | MELDLSPPHL SSSPEDLCPA PGTPPGTPRP PDTPLPEEVK RSQPLLIPTT GRKLREEERR ATSLPSIPNP FPELCSPPSQ SPILGGPSSA RGLLPRDASR PHVVKVYSED GACRSVEVAA GATARHVCEM LVQRAHALSD ETWGLVECHP HLALERGLED HESVVEVQAA WPVGGDSRFV FRKNFAKYEL FKSSPHSLFP EKMVSSCLDA HTGISHEDLI QNFLNAGSFP EIQGFLQLRG SGRKLWKRFF CFLRRSGLYY STKGTSKDPR HLQYVADVNE SNVYVVTQGR KLYGMPTDFG FCVKPNKLRN GHKGLRIFCS EDEQSRTCWL AAFRLFKYGV QLYKNYQQAQ SRHLHPSCLG SPPLRSASDN TLVAMDFSGH AGRVIENPRE ALSVALEEAQ AWRKKTNHRL SLPMPASGTS LSAAIHRTQL WFHGRISREE SQRLIGQQGL VDGLFLVRES QRNPQGFVLS LCHLQKVKHY LILPSEEEGR LYFSMDDGQT RFTDLLQLVE FHQLNRGILP CLLRHCCTRV AL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于Grb7重组蛋白的参考文献概览：
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1. **文献名称**：*Grb7 in intracellular signaling and its role in cell migration*
**作者**：Daly RJ et al.
**摘要**：研究揭示了Grb7重组蛋白在细胞迁移信号通路中的作用，通过SH2结构域与磷酸化受体(如EGFR)相互作用，促进肿瘤细胞侵袭。实验采用重组Grb7蛋白验证其与下游效应分子的结合机制。
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2. **文献名称**：*Structural basis for the interaction of the adaptor protein Grb7 with activated receptors*
**作者**：Pellicena P et al.
**摘要**：通过X射线晶体学解析了重组Grb7蛋白的SH2结构域与Her2受体磷酸化肽段的复合物结构，阐明了Grb7识别特定酪氨酸磷酸化位点的分子机制，为靶向抑制剂设计提供依据。
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3. **文献名称**：*Expression and purification of functional recombinant Grb7 protein for interaction studies*
**作者**：Chua BT et al.
**摘要**：报道了在大肠杆菌中高效表达可溶性Grb7重组蛋白的方法，并通过pull-down实验验证其与ErbB2受体的特异性结合，证明重组蛋白在体外信号通路研究中的适用性。
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**备注**：若需具体文献DOI或补充更多研究(如Grb7在癌症治疗中的应用)，可进一步说明方向。
Grb7 (Growth Factor Receptor-Bound Protein 7) is an adaptor protein involved in intracellular signaling pathways regulating cell proliferation, migration, and survival. It belongs to the Grb7/10/14 family, characterized by a conserved N-terminal proline-rich domain, a central pleckstrin homology (PH) domain, and a C-terminal Src homology 2 (SH2) domain. The SH2 domain enables Grb7 to bind phosphorylated tyrosine residues on activated receptor tyrosine kinases (RTKs), such as HER2. EGFR, and PDGFR, facilitating downstream signal transduction. This interaction links Grb7 to critical pathways like Ras-MAPK and PI3K/Akt, influencing cellular processes implicated in cancer progression and metastasis.
Grb7 overexpression is observed in various cancers, including breast, gastric, and pancreatic cancers, correlating with aggressive tumor behavior, poor prognosis, and therapeutic resistance. Its role in promoting cell migration and invasion has made it a potential therapeutic target. Recombinant Grb7 proteins are engineered for functional studies, drug screening, and structural analyses. Produced via bacterial (e.g., E. coli) or mammalian expression systems, these proteins retain key domains to study interactions with signaling partners or inhibitors. Purification methods often involve affinity chromatography (e.g., His-tag) followed by biochemical validation (e.g., Western blot, SPR).
Research on recombinant Grb7 has advanced understanding of its molecular mechanisms and guided inhibitor development, such as peptide-based or small-molecule compounds targeting its SH2 domain. However, challenges remain in achieving selective inhibition and translating findings into clinical applications. Ongoing studies focus on elucidating Grb7's isoform-specific functions and its crosstalk with other signaling hubs, aiming to refine therapeutic strategies against Grb7-driven malignancies.
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