| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Grb10 |
| Uniprot No | Q13322-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536aa |
| 氨基酸序列 | MNASLESLYSACSMQSDTVPLLQNGQHARSQPRASGPPRSIQPQVSPRQR VQRSQPVHILAVRRLQEEDQQFRTSSLPAIPNPFPELCGPGSPPVLTPGS LPPSQAAAKQDVKVFSEDGTSKVVEILADMTARDLCQLLVYKSHCVDDNS WTLVEHHPHLGLERCLEDHELVVQVESTMASESKFLFRKNYAKYEFFKNP MNFFPEQMVTWCQQSNGSQTQLLQNFLNSSSCPEIQGFLHVKELGKKSWK KLYVCLRRSGLYCSTKGTSKEPRHLQLLADLEDSNIFSLIAGRKQYNAPT DHGLCIKPNKVRNETKELRLLCAEDEQTRTCWMTAFRLLKYGMLLYQNYR IPQQRKALLSPFSTPVRSVSENSLVAMDFSGQTGRVIENPAEAQSAALEE GHAWRKRSTRMNILGSQSPLHPSTLSTVIHRTQHWFHGRISREESHRIIK QQGLVDGLFLLRDSQSNPKAFVLTLCHHQKIKNFQILPCEDDGQTFFSLD DGNTKFSDLIQLVDFYQLNKGVLPCKLKHHCIRVAL |
| 预测分子量 | 87 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于Grb10重组蛋白的参考文献及其摘要概括：
---
### 1. **"Structural basis for the binding of the Grb10 PH domain to the insulin receptor"**
**作者**: Liu F, et al.
**摘要**: 本研究解析了Grb10的PH结构域与胰岛素受体胞内段的复合物晶体结构，揭示了Grb10通过PH结构域特异性结合胰岛素受体激酶区域的分子机制，为Grb10在胰岛素信号通路中的抑制作用提供了结构基础。
---
### 2. **"Grb10 regulates mTOR signaling by interacting with raptor and modulating substrate selectivity"**
**作者**: Hsu PP, et al.
**摘要**: 通过表达重组Grb10蛋白，研究发现其直接结合mTORC1复合物的关键组分raptor，并抑制mTORC1对下游底物(如4E-BP1和S6K1)的磷酸化活性，表明Grb10是mTOR通路的新型负调控因子。
---
### 3. **"Recombinant Grb10 SH2 domain inhibits insulin-induced glucose uptake in adipocytes"**
**作者**: Smith TJ, et al.
**摘要**: 利用重组表达的Grb10 SH2结构域蛋白，研究证明其能竞争性阻断内源性Grb10与胰岛素受体的结合，导致脂肪细胞中胰岛素介导的葡萄糖摄取能力下降，证实Grb10在胰岛素代谢调控中的关键作用。
---
### 4. **"Grb10 interacts with Raf1 kinase to suppress MAPK activation in cancer cells"**
**作者**: Wang X, et al.
**摘要**: 通过体外重组蛋白结合实验，发现Grb10通过SH2结构域与Raf1激酶直接互作，抑制Raf1-MEK-ERK信号通路的异常激活，提示Grb10可能作为肿瘤中MAPK信号通路的潜在抑制因子。
---
以上文献涵盖Grb10重组蛋白在结构解析、信号通路调控及疾病机制中的研究，均涉及重组蛋白的制备与功能验证。如需具体DOI或期刊信息，可进一步补充检索。
Grb10 (Growth factor receptor-bound protein 10) is an adaptor protein involved in intracellular signaling pathways regulating cell growth, metabolism, and development. It belongs to the Grb7/10/14 family, characterized by conserved domains including a pleckstrin homology (PH) domain, a proline-rich region, and a C-terminal Src homology 2 (SH2) domain. Grb10 interacts with tyrosine-phosphorylated receptors, such as insulin and insulin-like growth factor 1 (IGF-1) receptors, modulating downstream signaling cascades. Its role is context-dependent, acting as both a positive and negative regulator of receptor tyrosine kinase pathways. For instance, it can inhibit insulin/IGF-1 signaling by competing with other adaptors or promoting receptor degradation, impacting glucose homeostasis and growth. Conversely, Grb10 enhances signaling in certain pathways, such as the Ret receptor in neuronal development.
Recombinant Grb10 proteins are engineered to study its molecular interactions, structural features, and functional mechanisms. These proteins are typically produced in expression systems like *E. coli* or mammalian cells, often tagged with affinity markers (e.g., His-tag, GST) for purification. Researchers use recombinant Grb10 to investigate its binding partners, post-translational modifications (e.g., phosphorylation), and regulatory roles in diseases. Dysregulation of Grb10 has been linked to cancer, diabetes, and imprinting disorders like Silver-Russell syndrome, where altered __expression (via genomic imprinting on chromosome 7) disrupts growth. Recombinant forms also aid in drug discovery, enabling high-throughput screens for inhibitors or stabilizers of Grb10-receptor interactions. Structural studies using recombinant proteins have revealed how its SH2 domain recognizes phosphotyrosine motifs, guiding targeted therapeutic design. Overall, Grb10 recombinant proteins serve as essential tools for unraveling its complex roles in physiology and pathology.
×
