| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAS6 |
| Uniprot No | Q14393-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-678aa |
| 氨基酸序列 | MAPSLSPGPAALRRAPQLLLLLLAAECALAALLPAREATQFLRPRQRRAF QVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYG SPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGG RLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDE CADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQV CVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRM FSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLAL RAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAV MKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNW LNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVG TESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKK QLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVS AAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNR RLLDLDEAAYKHSDITAHSCPPVEPAAA |
| 预测分子量 | 101 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAS6重组蛋白的3篇代表性文献，涵盖功能研究、结构解析及疾病应用方向：
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1. **标题**：*Recombinant GAS6 Signaling Attenuates Acute Kidney Injury in Mice by Modulating Macrophage Polarization*
**作者**：Li Y, et al.
**摘要**：研究通过重组GAS6蛋白处理小鼠急性肾损伤模型，发现其通过激活AXL受体促进巨噬细胞向抗炎表型转化，减轻炎症反应和肾小管细胞凋亡，提示GAS6可能成为治疗急性肾损伤的潜在靶点。
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2. **标题**：*Structural Basis of GAS6–AXL Signaling in Innate Immunity*
**作者**：Wang X, et al.
**摘要**：通过X射线晶体学解析了重组人源GAS6蛋白与AXL受体结合的复合物结构，揭示了GAS6的LG结构域与AXL的Ig1结构域的关键相互作用，为开发靶向GAS6/AXL通路的免疫调节药物提供结构基础。
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3. **标题**：*Recombinant GAS6 Enhances Cancer Cell Resistance to Chemotherapy via PI3K/Akt Pathway*
**作者**：Chen L, et al.
**摘要**：体外实验表明，重组GAS6通过激活肿瘤细胞中的PI3K/Akt信号通路，抑制化疗药物诱导的细胞凋亡，提示靶向GAS6/AXL轴可能改善癌症治疗的耐药性问题。
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**备注**：以上文献为示例，实际引用时需根据具体研究领域补充真实文献信息(可通过PubMed或Google Scholar搜索关键词“recombinant GAS6 protein”获取)。
Growth arrest-specific 6 (GAS6) is a vitamin K-dependent protein first identified in 1988 as a gene upregulated in growth-arrested fibroblasts. Structurally, it belongs to the same family as Protein S, featuring an N-terminal γ-carboxyglutamic acid (Gla) domain, four epidermal growth factor (EGF)-like repeats, and a C-terminal laminin G-like (LG) domain. These domains mediate critical interactions: the Gla domain binds phosphatidylserine on apoptotic cell membranes, while the LG domain engages TAM receptor tyrosine kinases (Tyro3. Axl, MerTK) to initiate downstream signaling.
GAS6 serves as a key regulator of cellular homeostasis, particularly in inflammation, apoptosis, and immune modulation. It promotes phagocytosis of apoptotic cells by macrophages, modulates platelet function, and influences vascular biology. Dysregulation of GAS6-TAM signaling is implicated in pathologies including cancer metastasis, thrombosis, fibrosis, and autoimmune disorders. In cancer, GAS6 often supports tumor progression by enhancing survival, invasion, and therapy resistance through TAM receptor activation.
Recombinant GAS6 proteins are produced using mammalian or insect expression systems to ensure proper post-translational modifications, particularly γ-carboxylation of the Gla domain required for membrane binding. These purified proteins are widely used to study TAM receptor biology, screen therapeutic agents targeting this pathway, or mimic physiological conditions in cell-based assays. Recent research also explores GAS6 as a potential biomarker or therapeutic target, with engineered variants being tested for modulating immune responses in cancer immunotherapy or tissue repair processes. Challenges remain in understanding context-dependent signaling outcomes and optimizing recombinant forms for clinical applications.
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