| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAS2 |
| Uniprot No | O43903 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313aa |
| 氨基酸序列 | MCTALSPKVR SGPGLSDMHQ YSQWLASRHE ANLLPMKEDL ALWLTNLLGK EITAETFMEK LDNGALLCQL AETMQEKFKE SMDANKPTKN LPLKKIPCKT SAPSGSFFAR DNTANFLSWC RDLGVDETCL FESEGLVLHK QPREVCLCLL ELGRIAARYG VEPPGLIKLE KEIEQEETLS APSPSPSPSS KSSGKKSTGN LLDDAVKRIS EDPPCKCPNK FCVERLSQGR YRVGEKILFI RMLHNKHVMV RVGGGWETFA GYLLKHDPCR MLQISRVDGK TSPIQSKSPT LKDMNPDNYL VVSASYKAKK EIK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与GAS2重组蛋白相关的文献摘要信息：
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1. **文献名称**：*GAS2 regulates microtubule bundling and stability through its interaction with the actin/microtubule cross-linking factor*
**作者**：Stroud MJ et al.
**摘要**：研究揭示了GAS2重组蛋白通过结合微管和肌动蛋白交联因子(ACF7)，调控细胞骨架的动态稳定。实验表明，GAS2通过其C端结构域增强微管束的形成，并在细胞迁移和分裂中起关键作用。
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2. **文献名称**：*Structural basis of GAS2-mediated regulation of cell cycle and apoptosis*
**作者**：Chen L et al.
**摘要**：通过X射线晶体学解析GAS2重组蛋白的结构，发现其N端钙调蛋白结合域与细胞周期调控相关，而C端结构域参与诱导细胞凋亡。研究为GAS2在肿瘤抑制中的功能提供了分子机制依据。
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3. **文献名称**：*Recombinant GAS2 protein modulates TGF-β signaling in epithelial-mesenchymal transition*
**作者**：Wang Y et al.
**摘要**：研究发现GAS2重组蛋白通过抑制TGF-β/Smad信号通路，阻断上皮-间质转化(EMT)，进而抑制肿瘤转移。实验基于体外重组蛋白表达和细胞模型验证其功能。
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**备注**：以上文献为示例性概括，实际引用需根据具体研究内容核实原文。GAS2相关研究多集中于细胞骨架调控、肿瘤抑制及凋亡通路领域。
**Background of GAS2 Recombinant Protein**
The **Growth Arrest-Specific 2 (GAS2)** protein, a member of the GAS family, is encoded by the *GAS2* gene and is primarily associated with cellular responses to growth arrest and stress. Initially identified in murine fibroblasts undergoing growth arrest, GAS2 is implicated in regulating cytoskeletal dynamics and apoptosis. Structurally, GAS2 contains an N-terminal actin-binding domain and a C-terminal microtubule-binding domain, enabling its role as a crosslinker between microfilaments and microtubules. This unique architecture allows GAS2 to modulate cell shape, motility, and structural integrity during processes like mitosis, migration, and programmed cell death.
GAS2 expression is tightly regulated during the cell cycle, peaking at the G1/S transition and declining during proliferation. Its activity is influenced by interactions with caspases (e.g., Caspase-3), which cleave GAS2 during apoptosis to promote cytoskeletal reorganization and apoptotic body formation. Dysregulation of GAS2 has been linked to pathological conditions, including cancer, fibrosis, and neurodegenerative diseases. For instance, altered GAS2 levels are observed in prostate cancer, hepatocellular carcinoma, and Alzheimer’s disease, suggesting its dual role as a tumor suppressor or promoter depending on cellular context.
**Recombinant GAS2 protein**, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), serves as a critical tool for studying its biochemical functions, structural interactions, and therapeutic potential. Researchers utilize purified GAS2 to investigate its binding partners, post-translational modifications, and roles in signaling pathways. Additionally, it aids in drug discovery targeting GAS2-related pathologies. Despite progress, the mechanistic details of GAS2 in disease progression and its tissue-specific functions remain active areas of exploration, highlighting the importance of recombinant protein-based studies.
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