首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GDF6 |
| Uniprot No | Q6KF10 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 336-455aa |
| 氨基酸序列 | TAFASRHGKR HGKKSRLRCS KKPLHVNFKE LGWDDWIIAP LEYEAYHCEG VCDFPLRSHL EPTNHAIIQT LMNSMDPGST PPSCCVPTKL TPISILYIDA GNNVVYKQYE DMVVESCGCR |
| 预测分子量 | 13.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GDF6重组蛋白的模拟参考文献示例，供参考(建议通过学术数据库核实具体文献)：
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1. **标题**: *GDF6 Recombinant Protein Promotes Chondrogenic Differentiation in Mesenchymal Stem Cells*
**作者**: Smith A, et al.
**摘要**: 本研究在大肠杆菌系统中表达了重组GDF6蛋白，并验证其生物活性。实验表明，GDF6显著促进间充质干细胞的软骨形成，提示其在关节修复中的潜在应用。
2. **标题**: *Structural and Functional Analysis of GDF6 in Zebrafish Embryogenesis*
**作者**: Lee B, Johnson R.
**摘要**: 通过重组GDF6蛋白的体外功能实验，揭示了其在斑马鱼胚胎骨骼发育中的关键作用，并解析了其与BMP受体结合的分子机制。
3. **标题**: *Optimized Production of Recombinant Human GDF6 in Mammalian Cells*
**作者**: Chen L, et al.
**摘要**: 报道了在HEK293细胞中高效表达和纯化重组人GDF6蛋白的工艺优化，验证了蛋白的稳定性及在成骨细胞分化中的功能活性。
4. **标题**: *GDF6 Mutations and Recombinant Protein Rescue in Craniofacial Development*
**作者**: Garcia S, et al.
**摘要**: 研究发现GDF6基因突变导致颅面发育异常，并通过重组GDF6蛋白回补实验证实其对颅骨软骨形成的调控作用。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“GDF6 recombinant protein”获取。
**Background of GDF6 Recombinant Protein**
Growth Differentiation Factor 6 (GDF6), also known as BMP13. is a member of the transforming growth factor-beta (TGF-β) superfamily, which plays critical roles in embryonic development, tissue differentiation, and homeostasis. GDF6 is particularly implicated in skeletal and joint development, regulating processes such as chondrogenesis, osteogenesis, and patterning of the axial skeleton. Studies have shown its involvement in specifying joint formation and maintaining cartilage integrity, with mutations linked to skeletal malformations (e.g., Klippel-Feil syndrome) and osteoarthritis.
Recombinant GDF6 protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to enable scalable and purified protein yields. Its production often requires careful optimization due to challenges like protein solubility, stability, and the need for proper post-translational modifications. The recombinant form retains bioactivity, mimicking native GDF6 by binding to BMP receptors (e.g., ALK3/6) and activating Smad-dependent signaling pathways.
Research applications of recombinant GDF6 include studying skeletal development, cartilage repair, and degenerative joint diseases. It is also explored in regenerative medicine for its potential to drive mesenchymal stem cell differentiation into chondrocytes or osteoblasts, aiding in tissue engineering strategies. However, its dual role in promoting or inhibiting bone formation, depending on context, underscores the need for precise dosing and delivery mechanisms.
Current studies focus on harnessing recombinant GDF6 for therapeutic interventions, such as enhancing fracture healing or mitigating cartilage degradation. Challenges remain in understanding its tissue-specific interactions and minimizing off-target effects. Advances in protein engineering and delivery systems may unlock its full potential in clinical applications.
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