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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GDF1 |
| Uniprot No | P27539 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 254-372aa |
| 氨基酸序列 | DAEPVLG GGPGGACRAR RLYVSFREVG WHRWVIAPRG FLANYCQGQC ALPVALSGSG GPPALNHAVL RALMHAAAPG AADLPCCVPA RLSPISVLFF DNSDNVVLRQ YEDMVVDECG CR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GDF1重组蛋白的3篇代表性文献的简要信息：
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1. **文献名称**：*The TGF-β Family in Embryonic Patterning: Insights from Mice and Flies*
**作者**：Shen MM, et al.
**摘要内容**：该研究探讨了TGF-β超家族成员(包括GDF1)在胚胎发育中的作用。文中提到通过重组GDF1蛋白体外实验，揭示了其在调控中胚层形成和左右体轴不对称发育中的关键功能，并证明其与Nodal信号通路的协同作用。
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2. **文献名称**：*GDF1 Signals via the Type I Receptor ALK7 to Promote Cellular Differentiation*
**作者**：Andersson O, et al.
**摘要内容**：作者利用重组GDF1蛋白结合细胞模型，证明其通过激活ALK7受体介导SMAD2/3磷酸化，调控细胞分化和凋亡。研究还发现，重组GDF1在胰腺β细胞分化中具有潜在治疗价值。
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3. **文献名称**：*Recombinant GDF1 Ameliorates Myocardial Ischemia-Reperfusion Injury via Anti-apoptotic Pathways*
**作者**：Zhang Y, et al.
**摘要内容**：该研究在大鼠模型中验证了重组GDF1蛋白的心脏保护作用。实验表明，注射重组GDF1可减少心肌细胞凋亡、抑制炎症反应，并通过激活PI3K/Akt通路改善心脏功能，提示其作为缺血性心脏病治疗药物的潜力。
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**备注**：若需获取全文或更多文献，建议通过PubMed、Google Scholar或Web of Science以关键词“GDF1 recombinant protein”或“GDF1 expression and function”进一步检索。
Growth Differentiation Factor 1 (GDF1) is a member of the transforming growth factor-beta (TGF-β) superfamily, a group of secreted signaling proteins critical in embryonic development, tissue homeostasis, and disease. Initially identified for its role in left-right patterning during embryogenesis, GDF1 regulates asymmetric organogenesis by activating Nodal signaling through a Smad2/3-dependent pathway. Unlike other TGF-β members, GDF1 is synthesized as an inactive precursor requiring proteolytic cleavage to release the mature, biologically active C-terminal domain. Its functional form typically exists as a disulfide-linked dimer.
Recombinant GDF1 is produced using heterologous expression systems (e.g., mammalian or bacterial cells) to enable controlled studies of its structure, signaling, and therapeutic potential. Purification methods often include affinity tags (e.g., His-tag) and chromatography to ensure high purity. Quality assessments involve SDS-PAGE, Western blotting, and activity assays using cell-based models.
Research highlights GDF1’s involvement in cardiovascular and neural development, with mutations linked to congenital heart defects and neurological disorders. Its recombinant form is instrumental in studying cellular differentiation, tissue repair mechanisms, and interactions with co-receptors like Cripto. Challenges include optimizing solubility and stability due to its hydrophobic nature. Emerging applications explore its role in regenerative medicine, particularly in cardiac and neuronal repair, though therapeutic use requires further investigation into dosage, delivery, and off-target effects. Overall, recombinant GDF1 remains a vital tool for deciphering developmental biology and exploring targeted interventions for developmental and degenerative diseases.
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