| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYF5 |
| Uniprot No | P13349 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 氨基酸序列 | MDVMDGCQFS PSEYFYDGSC IPSPEGEFGD EFVPRVAAFG AHKAELQGSD EDEHVRAPTG HHQAGHCLMW ACKACKRKST TMDRRKAATM RERRRLKKVN QAFETLKRCT TTNPNQRLPK VEILRNAIRY IESLQELLRE QVENYYSLPG QSCSEPTSPT SNCSDGMPEC NSPVWSRKSS TFDSIYCPDV SNVYATDKNS LSSLDCLSNI VDRITSSEQP GLPLQDLASL SPVASTDSQP ATPGASSSRL IYHVL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYF5重组蛋白的3篇参考文献的简要总结(注：文献为模拟示例，实际引用需核实具体来源)：
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1. **标题**: *"Recombinant MYF5 protein induces myogenic differentiation in pluripotent stem cells"*
**作者**: Smith A, et al.
**摘要**: 研究利用大肠杆菌表达系统成功纯化重组MYF5蛋白，并证明其能够通过激活肌肉特异性基因(如MyoD和肌球蛋白重链)，诱导多能干细胞向成肌细胞分化，为体外肌肉再生研究提供工具。
2. **标题**: *"Structural and functional analysis of MYF5 in skeletal muscle development"*
**作者**: Chen L, et al.
**摘要**: 通过晶体学解析MYF5重组蛋白的DNA结合结构域三维结构，揭示其与靶基因启动子结合的关键氨基酸残基，并利用体外实验验证其调控肌肉前体细胞增殖的功能。
3. **标题**: *"MYF5 recombinant protein enhances muscle repair in a murine injury model"*
**作者**: Gonzalez R, et al.
**摘要**: 在小鼠肌肉损伤模型中，局部注射重组MYF5蛋白显著加速卫星细胞活化和损伤修复，表明其在治疗肌肉萎缩疾病中的潜在应用价值。
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**提示**：实际研究中可查阅PubMed或Web of Science平台，以关键词“MYF5 recombinant protein”、“MYF5 expression and purification”或“MYF5 functional study”获取最新文献。
MYF5 (myogenic factor 5) is a crucial regulatory protein involved in skeletal muscle development and differentiation. As a member of the myogenic regulatory factor (MRF) family, which includes MyoD, Myogenin, and MRF4. MYF5 plays a pivotal role in initiating myogenesis during embryogenesis. It is one of the earliest markers expressed in myogenic progenitor cells within somites, directing their commitment to the muscle lineage. MYF5 functions by binding to specific DNA sequences to activate transcription of muscle-specific genes, coordinating with other MRFs and signaling pathways like Wnt and Shh.
Recombinant MYF5 protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., E. coli) or mammalian systems to ensure proper folding and biological activity. The purified protein retains the DNA-binding basic helix-loop-helix (bHLH) domain critical for its regulatory function. Researchers utilize recombinant MYF5 to study muscle cell differentiation mechanisms, model congenital myopathies, and explore regenerative therapies for muscular dystrophies or age-related muscle wasting (sarcopenia). Its application extends to in vitro reprogramming of stem cells into myoblasts and drug screening platforms targeting muscle disorders. Studies of MYF5 also provide insights into the evolutionary conservation of myogenesis across species. However, its functional redundancy with other MRFs and context-dependent activity in different developmental stages remain active research areas. As a research tool, recombinant MYF5 continues to bridge basic developmental biology with translational muscle regeneration strategies.
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