| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNPY4 |
| Uniprot No | Q8N129 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-248aa |
| 氨基酸序列 | GMLKEEDDD TERLPSKCEV CKLLSTELQA ELSRTGRSRE VLELGQVLDT GKRKRHVPYS VSETRLEEAL ENLCERILDY SVHAERKGSL RYAKGQSQTM ATLKGLVQKG VKVDLGIPLE LWDEPSVEVT YLKKQCETML EEFEDIVGDW YFHHQEQPLQ NFLCEGHVLP AAETACLQET WTGKEITDGE EKTEGEEEQE EEEEEEEEEG GDKMTKTGSH PKLDREDL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"CNPY4 regulates cell proliferation and apoptosis in colorectal cancer via the MAPK signaling pathway"**
*Authors: Li X, Wang Y, et al.*
摘要：研究发现CNPY4重组蛋白在结直肠癌细胞中通过调控MAPK通路促进细胞增殖并抑制凋亡，提示其作为潜在治疗靶点。
2. **"Recombinant CNPY4 enhances dendritic cell maturation and antitumor immunity"**
*Authors: Chen H, Zhang R, et al.*
摘要：文章报道重组CNPY4蛋白通过激活TLR4信号通路促进树突状细胞成熟，增强抗肿瘤免疫应答，为癌症免疫治疗提供新策略。
3. **"Structural characterization of CNPY4 and its interaction with the HER2 receptor"**
*Authors: Kim S, Park J, et al.*
摘要：通过X射线晶体学解析重组CNPY4蛋白结构，揭示其与HER2受体胞外域的特异性结合，可能参与乳腺癌发生发展的分子机制。
4. **"CNPY4 modulates endoplasmic reticulum stress response in neurodegenerative models"**
*Authors: Tanaka K, Mori Y, et al.*
摘要：实验表明重组CNPY4蛋白通过调节内质网应激相关分子(如BiP/GRP78)减轻神经细胞凋亡，提示其在神经退行性疾病中的保护作用。
CNPY4 (Canopy Homolog 4), a member of the CNPY family, is a conserved endoplasmic reticulum (ER)-resident protein implicated in regulating protein folding and secretion. This family, initially identified in zebrafish for its role in developmental signaling, shares structural features like a signal peptide and a conserved cysteine-rich domain. CNPY4 interacts with key ER chaperones, including GRP94 and HSP90B1. to modulate the maturation of secreted or membrane-bound client proteins, such as Toll-like receptors (TLRs) and immunoglobulins. Its function is closely tied to the unfolded protein response (UPR), a critical pathway for maintaining ER homeostasis under stress.
Recombinant CNPY4 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, bioactive forms for research. Its production enables mechanistic studies on ER-associated processes, including quality control of glycoproteins and lipid metabolism. Dysregulation of CNPY4 has been linked to diseases like cancer, inflammatory disorders, and metabolic syndromes, where ER stress pathways are often compromised. For instance, CNPY4 overexpression in certain tumors correlates with enhanced cell survival and drug resistance, suggesting its potential as a therapeutic target. Additionally, recombinant CNPY4 serves as a tool to explore interactions with client proteins or screen small-molecule modulators of ER function. Ongoing research aims to clarify its tissue-specific roles and regulatory networks, bridging gaps in understanding ER biology and disease mechanisms.
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