| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPNT |
| Uniprot No | Q9Y639 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-220aa |
| 氨基酸序列 | QNEPRIVTSEEVIIRDSPVLPVTLQCNLTSSSHTLTYSYWTKNGVELSAT RKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKAAPDITGHK RSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINK ENYTELNIVNLQITEDPGEYECNATNAIGSASVVTVLRVRSHVDHHHHHH |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与NPNT(Nephronectin)重组蛋白相关的研究文献摘要信息，供参考：
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1. **文献名称**: *"Recombinant Nephronectin Promotes Osteoblast Differentiation via Integrin α8β1"*
**作者**: Smith J, et al.
**摘要**: 该研究利用重组NPNT蛋白探究其对成骨细胞分化的调控作用，发现其通过结合细胞表面整合素α8β1激活下游信号通路，促进成骨相关基因表达，为骨组织再生提供潜在治疗策略。
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2. **文献名称**: *"Production and Functional Characterization of Human Recombinant Nephronectin in E. coli"*
**作者**: Tanaka R, et al.
**摘要**: 研究报道了一种在大肠杆菌中高效表达重组人源NPNT蛋白的方法，并通过体外细胞黏附实验验证其生物活性，证明其能显著增强肾小管上皮细胞的黏附能力。
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3. **文献名称**: *"NPNT-mediated Wnt/β-catenin Signaling Drives Liver Regeneration"*
**作者**: Chen L, et al.
**摘要**: 研究发现重组NPNT蛋白通过激活Wnt/β-catenin通路促进肝细胞增殖，在小鼠肝部分切除模型中加速组织修复，揭示了NPNT在肝脏再生中的关键作用。
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**备注**：以上文献信息为示例性内容，实际文献需通过PubMed或Web of Science等数据库检索。建议结合具体研究方向(如骨再生、癌症或器官发育)进一步筛选。
**Background of Recombinant NPNT Protein**
Nephronectin (NPNT) is an extracellular matrix (ECM) protein that plays critical roles in tissue development, cell adhesion, and organogenesis. It was initially identified for its essential function in kidney development, particularly in the formation of the glomerular basement membrane and tubulogenesis. Structurally, NPNT contains multiple functional domains, including EGF-like repeats and an RGD (Arg-Gly-Asp) motif, which mediates cell adhesion by binding to integrin receptors such as α8β1 and αvβ3. These interactions facilitate cell-ECM communication, influencing cellular behaviors like migration, proliferation, and differentiation.
The recombinant form of NPNT is produced using biotechnological platforms, such as mammalian or bacterial expression systems, to ensure proper folding and post-translational modifications. Recombinant NPNT retains its bioactive properties, making it a valuable tool for studying ECM biology, tissue engineering, and regenerative medicine. It has shown promise in promoting osteogenesis, angiogenesis, and wound healing, attributed to its ability to modulate integrin-mediated signaling pathways.
In research, recombinant NPNT is utilized to investigate its role in diseases, including chronic kidney disease, cancer, and fibrosis, where dysregulation of ECM components contributes to pathology. For instance, NPNT overexpression in certain cancers is linked to tumor progression and metastasis, while its deficiency is associated with renal abnormalities. Additionally, its application in biomaterial coatings enhances the biocompatibility of implants by encouraging cell attachment and tissue integration.
Overall, recombinant NPNT serves as both a therapeutic candidate and a research reagent, bridging insights into developmental biology and translational applications in regenerative therapies and disease modeling.
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