| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSP |
| Uniprot No | O95405 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1425aa |
| 氨基酸序列 | MENYFQAEAYNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPPSHRLSFNPTLASVNESAVSNESQPQLKVFSLAHSAPLTTEEEDHCANGQDCNLNPEIATMWIDENAVAEDQLIKRNYSWDDQCSAVEVGEKKCGNLACLPDEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQSLMDAFSCSLDNENRQTDQFSFSINESTEKDMNSEKQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSPSQLKDDGSIGRDPSMSAITSLTVDSVISSQGTDGCPAVKKQENYIPDEDLTGKISSPRTDLGSPNSFSHMSEGILMKKEPAEESTTEESLRSGLPLLLKPDMPNGSGRNNDCERCSDCLVPNEVRADENEGYEHEETLGTTEFLNMTEHFSESQDMTNWKLTKLNEMNDSQVNEEKEKFLQISQPEDTNGDSGGQCVGLADAGLDLKGTCISESEECDFSTVIDTPAANYLSNGCDSYGMQDPGVSFVPKTLPSKEDSVTEEKEIEESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMKKNYLHNFCSQVPSVLGQSSPKVVASLPSISVPFGGARPKQPSNLKLQIPKPLSDHLQNDFPANSGNNTKNKNDILGKAKLGENSATNVCSPSLGNISNVDTNGEHLESYEAEISTRPCLALAPDSPDNDLRAGQFGISARKPFTTLGEVAPVWVPDSQAPNCMKCEARFTFTKRRHHCRACGKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNAQAWENMMSASSQSPNPNNPAEYCSTIPPLQQAQASGALSSPPPTVMVPVGVLKHPGAEVAQPREQRRVWFADGILPNGEVADAAKLTMNGTSSAGTLAVSHDPVKPVTTSPLPAETDICLFSGSITQVGSPVGSAMNLIPEDGLPPILISTGVKGDYAVEEKPSQISVMQQLEDGGPDPLVFVLNANLLSMVKIVNYVNRKCWCFTTKGMHAVGQSEIVILLQCLPDEKCLPKDIFNHFVQLYRDALAGNVVSNLGHSFFSQSFLGSKEHGGFLYVTSTYQSLQDLVLPTPPYLFGILIQKWETPWAKVFPIRLMLRLGAEYRLYPCPLFSVRFRKPLFGETGHTIMNLLADFRNYQYTLPVVQGLVVDMEVRKTSIKIPSNRYNEMMKAMNKSNEHVLAGGACFNEKADSHLVCVQNDDGNYQTQAISIHNQPRKVTGASFFVFSGALKSSSGYLAKSSIVEDGVMVQITAENMDSLRQALREMKDFTITCGKADAEEPQEHIHIQWVDDDKNVSKGVVSPIDGKSMETITNVKIFHGSEYKANGKVIRWTEVFFLENDDQHNCLSDPADHSRLTEHVAKAFCLALCPHLKLLKEDGMTKLGLRVTLDSDQVGYQAGSNGQPLPSQYMNDLDSALVPVIHGGACQLSEGPVVMELIFYILENIV |
| 预测分子量 | 156,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NSP重组蛋白的3篇示例参考文献(内容为虚构，仅供格式参考)：
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1. **文献名称**：*Expression and Functional Characterization of SARS-CoV-2 NSP12 RNA-Dependent RNA Polymerase*
**作者**：Smith, J.R. et al.
**摘要**：研究利用昆虫细胞系统成功表达并纯化了SARS-CoV-2的NSP12重组蛋白，证实其与辅助因子NSP7/NSP8协同作用时可显著增强RNA聚合酶活性，为靶向抗病毒药物设计提供依据。
2. **文献名称**：*Structural Insights into NSP5 Main Protease of SARS-CoV-2 Using Cryo-EM*
**作者**：Wang, Y. et al.
**摘要**：通过冷冻电镜解析了NSP5重组蛋白的三维结构，揭示了其底物结合口袋的关键氨基酸残基，并筛选出小分子抑制剂可有效抑制其蛋白酶活性，为COVID-19治疗策略提供新方向。
3. **文献名称**：*NSP1 as a Viral Immune Evasion Tool: Interactions with Host Ribosomes*
**作者**：Garcia, M.P. et al.
**摘要**：研究在大肠杆菌中重组表达了SARS-CoV-2的NSP1蛋白，发现其通过结合宿主核糖体抑制mRNA翻译，并阻断干扰素信号通路，阐明了NSP1在病毒免疫逃逸中的关键作用。
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*注：以上文献为示例，实际引用时请查阅真实学术数据库(如PubMed、Web of Science)获取准确信息。*
**Background of NSP Recombinant Proteins**
Non-structural proteins (NSPs) are critical components encoded by viral genomes, particularly in RNA viruses such as coronaviruses, picornaviruses, and flaviviruses. Unlike structural proteins that form the viral particle, NSPs primarily facilitate viral replication, transcription, and immune evasion by interacting with host cellular machinery. For instance, in coronaviruses like SARS-CoV-2. NSPs (e.g., NSP3. NSP5. NSP12) form the replication-transcription complex (RTC), enabling RNA synthesis and processing.
Recombinant NSPs are engineered through genetic cloning and expression in heterologous systems (e.g., *E. coli*, yeast, or mammalian cells*), allowing large-scale production for functional studies. These proteins are pivotal in dissecting viral life cycles, identifying therapeutic targets, and developing antivirals. For example, NSP5 (3CL protease) is a key drug target due to its role in polyprotein processing.
The production of recombinant NSPs faces challenges, including proper folding, post-translational modifications, and solubility. Advanced systems like mammalian or insect cell cultures are often employed to address these issues, ensuring biologically active conformations. Purification techniques (e.g., affinity chromatography) further enhance yield and purity.
Beyond basic research, recombinant NSPs are used in diagnostic assays (e.g., ELISA) to detect antiviral antibodies and in structural biology (e.g., cryo-EM, X-ray crystallography) to resolve 3D architectures for drug design. Recent advancements in synthetic biology and computational modeling have accelerated their application in personalized vaccines and broad-spectrum therapeutics.
In summary, recombinant NSPs serve as indispensable tools for understanding viral pathogenesis and advancing biomedical interventions, bridging molecular virology with translational innovation.
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