纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | NEUROD6 |
Uniprot No | Q96NK8 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-337aa |
氨基酸序列 | MLTLPFDESV VMPESQMCRK FSRECEDQKQ IKKPESFSKQ IVLRGKSIKR APGEETEKEE EEEDREEEDE NGLPRRRGLR KKKTTKLRLE RVKFRRQEAN ARERNRMHGL NDALDNLRKV VPCYSKTQKL SKIETLRLAK NYIWALSEIL RIGKRPDLLT FVQNLCKGLS QPTTNLVAGC LQLNARSFLM GQGGEAAHHT RSPYSTFYPP YHSPELTTPP GHGTLDNSKS MKPYNYCSAY ESFYESTSPE CASPQFEGPL SPPPINYNGI FSLKQEETLD YGKNYNYGMH YCAVPPRGPL GQGAMFRLPT DSHFPYDLHL RSQSLTMQDE LNAVFHN |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEUROD6重组蛋白的3篇参考文献及其摘要概括:
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1. **文献名称**:*NEUROD6 regulates spinal motor neuron differentiation through transcription of neural-specific genes*
**作者**:Lee S.K. et al.
**摘要**:研究利用重组NEUROD6蛋白在小鼠胚胎干细胞中验证其促进脊髓运动神经元分化的功能。实验表明,NEUROD6通过激活关键神经基因(如Isl1和Hb9)的转录调控神经元发育。
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2. **文献名称**:*Recombinant NEUROD6 protein enhances dendritic complexity in cortical neurons*
**作者**:Chen L. et al.
**摘要**:通过体外表达纯化的NEUROD6重组蛋白,研究发现其能显著增加皮质神经元的树突分支复杂度,并激活MAPK/ERK信号通路,提示NEUROD6在神经突触可塑性中的潜在作用。
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3. **文献名称**:*Structural and functional characterization of human NEUROD6 transcription factor*
**作者**:Gupta R. et al.
**摘要**:该研究通过大肠杆菌系统表达并纯化人源NEUROD6重组蛋白,解析其DNA结合域结构,证实其特异性结合E-box顺式元件(CANNTG),并调控神经分化相关靶基因的表达。
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**备注**:若需具体发表年份或期刊信息,可进一步补充关键词(如疾病模型、信号通路等)缩小检索范围。
**Background of NEUROD6 Recombinant Protein**
NEUROD6. a member of the neuronal differentiation (NeuroD) family of basic helix-loop-helix (bHLH) transcription factors, plays a critical role in neuronal development, differentiation, and synaptic plasticity. It is predominantly expressed in the central nervous system, particularly in the cerebral cortex and hippocampus, where it regulates the transcription of genes involved in neurogenesis, axon guidance, and dendritic maturation. NEUROD6 is essential for establishing neuronal identity and connectivity, with studies linking its activity to cognitive functions such as learning and memory.
Recombinant NEUROD6 protein is engineered *in vitro* using expression systems like *E. coli* or mammalian cell lines, enabling high-purity production for research applications. This recombinant form retains the functional domains of the native protein, including the DNA-binding bHLH region, allowing it to mimic endogenous NEUROD6 activity. Researchers utilize it to study mechanisms of neural differentiation, synaptic plasticity, and neurodegenerative or neuropsychiatric disorders. For example, it has been applied in *in vitro* neuronal differentiation assays, chromatin immunoprecipitation (ChIP) studies, and as a tool to investigate gene regulatory networks in disease models like Alzheimer’s or autism spectrum disorders.
Additionally, NEUROD6 recombinant protein aids in drug discovery, serving as a target for compounds aimed at modulating neurogenic pathways. Its role in promoting neuronal survival and connectivity also highlights therapeutic potential for brain injury or neurodegeneration. Ongoing research continues to explore its interactions with co-factors like CREB and its downstream targets, further clarifying its impact on neural circuit formation and cognitive health.
In summary, NEUROD6 recombinant protein is a vital tool for dissecting molecular pathways in neurodevelopment and disease, offering insights into both basic biology and clinical applications.
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