| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPTX1 |
| Uniprot No | Q15818 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 88-227aa |
| 氨基酸序列 | RCESQSTLDPGAGEARAGGGRKQPGSGKNTMGDLSRTPAAETLSQLGQTLQSLKTRLENLEQYSRLNSSSQTNSLKDLLQSKIDELERQVLSRVNTLEEGKGGPRNDTEERVKIETALTSLHQRISELEKGQKDNRPGDK |
| 预测分子量 | 50.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPTX1重组蛋白的模拟参考文献示例(请注意，这些文献为虚构示例，仅供格式参考，实际文献请通过学术数据库查询)：
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1. **文献名称**: Recombinant Expression and Functional Characterization of NPTX1 in Synaptic Plasticity
**作者**: Smith A, et al.
**摘要**: 研究报道了在大肠杆菌中成功表达并纯化重组NPTX1蛋白，通过体外神经元培养实验证实其能够增强突触密度和信号传递，提示其在神经可塑性中的调节作用。
2. **文献名称**: Structural Insights into NPTX1 Pentamer Assembly and Its Role in Neurodegenerative Diseases
**作者**: Zhang L, et al.
**摘要**: 利用重组NPTX1蛋白解析了其五聚体晶体结构，并发现其与淀粉样蛋白β的相互作用可能影响阿尔茨海默病的病理进程。
3. **文献名称**: Development of a High-Yield NPTX1 Recombinant Protein Production System in Mammalian Cells
**作者**: Wang Q, et al.
**摘要**: 构建了基于HEK293细胞的重组NPTX1高效表达系统，优化纯化工艺后获得具有生物活性的蛋白，为后续功能研究提供可靠工具。
4. **文献名称**: NPTX1 Recombinant Protein Attenuates Neuroinflammation via Modulating Microglial Activation
**作者**: Johnson R, et al.
**摘要**: 实验表明，重组NPTX1蛋白通过抑制小胶质细胞TLR4/NF-κB通路，减少炎症因子释放，为治疗神经炎症疾病提供潜在策略。
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**建议**：如需真实文献，可通过以下关键词在PubMed或Google Scholar检索：
- "NPTX1 recombinant protein expression"
- "NPTX1 structure/function"
- "NPTX1 neuronal signaling"
**Background of NPTX1 Recombinant Protein**
Neuronal pentraxin 1 (NPTX1), a member of the long pentraxin subfamily, is a secreted glycoprotein implicated in synaptic plasticity and neuronal connectivity. Pentraxins are evolutionarily conserved proteins characterized by a unique “pentraxin domain,” which facilitates ligand binding and protein interactions. NPTX1 is primarily expressed in the central nervous system (CNS), where it modulates excitatory synapse function by regulating the clustering of AMPA-type glutamate receptors. Its structure includes a conserved N-terminal pentraxin domain and a C-terminal region that enables oligomerization, typically forming an octameric complex.
Recombinant NPTX1 protein is engineered using expression systems like mammalian cells (e.g., HEK293) or *E. coli*, ensuring proper folding and post-translational modifications critical for its biological activity. Purification involves affinity chromatography, yielding high-purity protein for research applications. Studies highlight its role in neurodevelopmental and neurodegenerative disorders, such as Alzheimer’s disease, where dysregulated NPTX1 expression correlates with synaptic dysfunction. Additionally, NPTX1 has been linked to cancer progression, acting as a potential biomarker or therapeutic target due to its involvement in cell adhesion and migration.
As a research tool, recombinant NPTX1 aids in elucidating mechanisms of synaptic transmission, neuronal repair, and disease pathways. It also serves in antibody production, drug screening, and diagnostic assay development. Despite its established roles, further studies are needed to fully unravel its interactions with other pentraxins (e.g., NPTXR/NPTX2) and its broader implications in cellular signaling and pathology.
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