首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDNF |
| Uniprot No | Q8TB73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-568aa |
| 氨基酸序列 | Q KLPTRDEELF QMQIRDKAFF HDSSVIPDGA EISSYLFRDT PKRYFFVVEE DNTPLSVTVT PCDAPLEWKL SLQELPEDRS GEGSGDLEPL EQQKQQIINE EGTELFSYKG NDVEYFISSS SPSGLYQLDL LSTEKDTHFK VYATTTPESD QPYPELPYDP RVDVTSLGRT TVTLAWKPSP TASLLKQPIQ YCVVINKEHN FKSLCAVEAK LSADDAFMMA PKPGLDFSPF DFAHFGFPSD NSGKERSFQA KPSPKLGRHV YSRPKVDIQK ICIGNKNIFT VSDLKPDTQY YFDVFVVNIN SNMSTAYVGT FARTKEEAKQ KTVELKDGKI TDVFVKRKGA KFLRFAPVSS HQKVTFFIHS CLDAVQIQVR RDGKLLLSQN VEGIQQFQLR GKPKAKYLVR LKGNKKGASM LKILATTRPT KQSFPSLPED TRIKAFDKLR TCSSATVAWL GTQERNKFCI YKKEVDDNYN EDQKKREQNQ CLGPDIRKKS EKVLCKYFHS QNLQKAVTTE TIKGLQPGKS YLLDVYVIGH GGHSVKYQSK VVKTRKFC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NDNF(Neuron-Derived Neurotrophic Factor)重组蛋白的参考文献示例(注：部分文献信息为假设性示例，实际文献需通过学术数据库验证)：
---
1. **文献名称**：*Recombinant NDNF promotes angiogenesis and protects against ischemic injury in a mouse model*
**作者**：Zhang, L., et al.
**摘要**：本研究通过大肠杆菌表达系统成功制备了重组NDNF蛋白，验证其生物活性。实验表明，重组NDNF能显著增强内皮细胞迁移和血管形成，并在小鼠后肢缺血模型中改善血流恢复，提示其作为缺血性疾病的潜在治疗靶点。
---
2. **文献名称**：*NDNF重组蛋白通过激活Akt信号通路抑制神经元凋亡*
**作者**：Wang, Y., et al.
**摘要**：该文献利用HEK293细胞表达纯化重组NDNF蛋白，探究其对氧化应激诱导的神经元凋亡的保护机制。结果显示，NDNF通过激活Akt磷酸化抑制Caspase-3活性，显著减少海马神经元凋亡，为神经退行性疾病治疗提供新思路。
---
3. **文献名称**：*Structural and functional characterization of recombinant NDNF: Implications for synaptic plasticity*
**作者**：Smith, J.R., et al.
**摘要**：通过X射线晶体学解析重组NDNF的三维结构，结合体外实验证明其与TrkB受体的相互作用。体内实验发现，NDNF重组蛋白可增强小鼠海马区突触可塑性，改善学习记忆能力，支持其在认知障碍疾病中的应用潜力。
---
4. **文献名称**：*Optimization of NDNF recombinant protein production in Pichia pastoris for clinical translation*
**作者**：Chen, H., et al.
**摘要**：本研究在毕赤酵母系统中优化NDNF重组蛋白的分泌表达条件，获得高纯度产物。药代动力学分析显示其半衰期延长，且无明显免疫原性，为后续临床试验奠定基础。
---
**提示**：以上文献为示例，实际研究中建议通过PubMed、Google Scholar等平台以关键词“NDNF recombinant protein”或“Neuron-Derived Neurotrophic Factor expression”检索最新成果，并优先选择高影响力期刊(如*Nature Communications*、*PNAS*等)的文献。
Neuron-Derived Neurotrophic Factor (NDNF) is a secreted protein first identified in 2011 as a member of the neurotrophic factor family. It plays critical roles in neuronal survival, synaptic plasticity, and vascular development. Structurally, NDNF contains conserved domains homologous to clotting factors and neurotrophic proteins, enabling interactions with extracellular matrix components and cell surface receptors like integrins. Its expression is prominent in the brain, particularly in the cerebral cortex, hippocampus, and cerebellum, but also detected in non-neural tissues, suggesting pleiotropic functions.
Recombinant NDNF production emerged to address the limitations of native protein isolation, such as low abundance and purification complexity. Using eukaryotic expression systems (e.g., HEK293 or CHO cells), researchers produce glycosylated NDNF with proper folding and bioactivity. Alternatively, bacterial systems yield non-glycosylated versions for structural studies. The recombinant protein has become essential for functional studies, revealing NDNF's neuroprotective effects in Alzheimer’s and Parkinson’s disease models, its role in angiogenesis, and potential therapeutic value in ischemic injury.
Current research focuses on optimizing delivery methods (e.g., viral vectors, nanoparticles) and exploring its dual neurovascular regulatory mechanisms. Challenges include understanding tissue-specific receptor interactions and improving blood-brain barrier penetration. As a relatively new target, recombinant NDNF continues to drive discoveries in neurodegeneration, cardiovascular diseases, and regenerative medicine.
×
