| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPAS4 |
| Uniprot No | Q8IUM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-802aa |
| 氨基酸序列 | MYRSTKGASKARRDQINAEIRNLKELLPLAEADKVRLSYLHIMSLACIYTRKGVFFAGGTPLAGPTGLLSAQELEDIVAALPGFLLVFTAEGKLLYLSESVSEHLGHSMVDLVAQGDSIYDIIDPADHLTVRQQLTLPSALDTDRLFRCRFNTSKSLRRQSAGNKLVLIRGRFHAHPPGAYWAGNPVFTAFCAPLEPRPRPGPGPGPGPASLFLAMFQSRHAKDLALLDISESVLIYLGFERSELLCKSWYGLLHPEDLAHASAQHYRLLAESGDIQAEMVVRLQAKTGGWAWIYCLLYSEGPEGPITANNYPISDMEAWSLRQQLNSEDTQAAYVLGTPTMLPSFPENILSQEECSSTNPLFTAALGAPRSTSFPSAPELSVVSASEELPRPSKELDFSYLTFPSGPEPSLQAELSKDLVCTPPYTPHQPGGCAFLFSLHEPFQTHLPTPSSTLQEQLTPSTATFSDQLTPSSATFPDPLTSPLQGQLTETSVRSYEDQLTPCTSTFPDQLLPSTATFPEPLGSPAHEQLTPPSTAFQAHLDSPSQTFPEQLSPNPTKTYFAQEGCSFLYEKLPPSPSSPGNGDCTLLALAQLRGPLSVDVPLVPEGLLTPEASPVKQSFFHYSEKEQNEIDRLIQQISQLAQGMDRPFSAEAGTGGLEPLGGLEPLDSNLSLSGAGPPVLSLDLKPWKCQELDFLADPDNMFLEETPVEDIFMDLSTPDPSEEWGSGDPEAEGPGGAPSPCNNLSPEDHSFLEDLATYETAFETGVSAFPYDGFTDELHQLQSQVQDSFHEDGSGGEPTF |
| 预测分子量 | 87,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPAS4重组蛋白的参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*NPAS4 regulates excitatory-inhibitory balance in neuronal networks through activity-dependent gene expression*
**作者**：Lin Y., et al.
**摘要**：研究利用重组NPAS4蛋白，揭示了其通过结合特定DNA序列调控突触可塑性相关基因的机制，并证明其在维持神经元网络兴奋-抑制平衡中的关键作用。
2. **文献名称**：*Structural and functional analysis of recombinant NPAS4 in hypoxia response*
**作者**：Kim S., Zhang H.
**摘要**：通过大肠杆菌表达系统获得重组NPAS4蛋白，解析其与HIF-1α的相互作用结构，阐明其在缺氧条件下调控神经保护基因的分子基础。
3. **文献名称**：*Recombinant NPAS4 as a therapeutic target for neurodegenerative disorders*
**作者**：Chen L., et al.
**摘要**：研究发现重组NPAS4蛋白可激活抗氧化通路，减轻阿尔茨海默病模型中的神经元损伤，提示其作为神经退行性疾病治疗靶点的潜力。
4. **文献名称**：*In vitro reconstitution of NPAS4-ARNT2 heterodimerization using recombinant proteins*
**作者**：Michaels R., et al.
**摘要**：利用重组NPAS4和ARNT2蛋白在体外重建异源二聚体复合物，通过生化实验验证二者结合的特异性及对下游基因启动子的调控功能。
(注：以上文献及内容为示例，实际文献请通过PubMed、Google Scholar等平台查询。)
NPAS4 (neuronal PAS domain protein 4) is an activity-dependent transcription factor belonging to the basic helix-loop-helix (bHLH)-PAS family. It plays a critical role in coupling neuronal activity to gene expression, particularly in excitatory neurons. Discovered in 2005. NPAS4 is rapidly induced by synaptic activity, calcium signaling, and neurotrophic factors, acting as a master regulator of inhibitory synapse development and homeostatic plasticity. Its structure includes a bHLH domain for DNA binding and dimerization, and PAS domains for protein-protein interactions, notably with ARNT (aryl hydrocarbon receptor nuclear translocator) to form functional complexes.
This protein is uniquely expressed in the brain, with prominent roles in learning, memory formation, and experience-dependent plasticity. NPAS4 regulates downstream genes such as BDNF (brain-derived neurotrophic factor) and proteins involved in GABAergic synapse formation. Its activity modulates the balance between excitatory and inhibitory neurotransmission, making it crucial for preventing network hyperexcitability and seizure susceptibility.
Recombinant NPAS4 protein is produced using expression systems like E. coli or mammalian cells, often fused with tags (e.g., His-tag) for purification via affinity chromatography. Researchers employ it to study neurodevelopmental pathways, screen neuroactive compounds, and model neurological disorders. Dysregulation of NPAS4 has been implicated in autism spectrum disorders, schizophrenia, and neurodegenerative diseases, positioning it as a potential therapeutic target. Current studies focus on its role in stress responses, circadian rhythms, and synaptic scaling mechanisms, with recombinant versions enabling precise biochemical and structural analyses.
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