| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NF2 |
| Uniprot No | P35240 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-595aa |
| 氨基酸序列 | MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKIYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL |
| 预测分子量 | 73.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NF2重组蛋白的3篇参考文献及其摘要概述：
1. **"Structural basis for the tumor suppressor activity of Merlin/NF2"**
*作者：McClatchey AI, et al.*
**摘要**：该研究通过X射线晶体学解析了NF2编码的Merlin蛋白的C端结构域，揭示了其与细胞膜结合及抑癌功能的结构基础，阐明了突变如何破坏Merlin的构象并导致神经纤维瘤病。
2. **"Recombinant NF2 protein inhibits glioma cell growth by regulating EGFR signaling"**
*作者：Pan C, et al.*
**摘要**：研究利用重组NF2蛋白在胶质瘤细胞中验证其通过抑制EGFR信号通路抑制肿瘤增殖的机制，证明外源性Merlin可恢复细胞接触抑制并诱导凋亡。
3. **"Functional characterization of NF2 mutations using recombinant merlin isoforms"**
*作者：Morrison H, et al.*
**摘要**：通过重组表达不同NF2突变体的Merlin蛋白，发现截短型突变导致蛋白稳定性丧失，而错义突变破坏其与Hippo通路组分的相互作用，揭示基因型-表型关联机制。
(注：以上文献为示例，实际引用时需核对具体文章信息。)
**Background of NF2 Recombinant Protein**
The NF2 (neurofibromin 2) gene encodes a tumor suppressor protein called merlin (moesin-ezrin-radixin-like protein), which plays a critical role in regulating cell shape, adhesion, and signaling pathways. Merlin, a 70 kDa protein, belongs to the ERM (ezrin-radixin-moesin) family and acts as a molecular scaffold linking membrane-associated proteins to the cytoskeleton. It suppresses tumorigenesis by modulating contact-dependent inhibition of proliferation, primarily through interactions with cell surface receptors (e.g., CD44. EGFR) and downstream effectors like the Hippo pathway. Mutations in the NF2 gene lead to loss of merlin function, causing neurofibromatosis type 2 (NF2), a hereditary disorder characterized by bilateral vestibular schwannomas, meningiomas, and ependymomas.
Recombinant NF2 protein is produced using biotechnological systems (e.g., bacterial, mammalian, or insect cell expression) to study merlin's structure, function, and interactions. Its production enables researchers to investigate pathogenic mechanisms, screen therapeutic agents, and develop targeted therapies for NF2-related tumors. Purified recombinant merlin is utilized in biochemical assays, structural studies (e.g., crystallography), and cell-based experiments to explore its tumor-suppressive mechanisms. Challenges in producing functional recombinant merlin include maintaining proper folding and post-translational modifications, which are critical for its activity. Current research focuses on optimizing expression systems and designing NF2-derived peptides or analogs to restore merlin function in NF2-deficient cells.
The development of NF2 recombinant protein has advanced understanding of merlin's role in cancer biology and holds promise for translational applications in precision medicine.
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