| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCAM2 |
| Uniprot No | O15394-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-697aa |
| 氨基酸序列 | MSLLLSFYLL GLLVSSGQAL LQVTISLSKV ELSVGESKFF TCTAIGEPES IDWYNPQGEK IISTQRVVVQ KEGVRSRLTI YNANIEDAGI YRCQATDAKG QTQEATVVLE IYQKLTFREV VSPQEFKQGE DAEVVCRVSS SPAPAVSWLY HNEEVTTISD NRFAMLANNN LQILNINKSD EGIYRCEGRV EARGEIDFRD IIVIVNVPPA ISMPQKSFNA TAERGEEMTF SCRASGSPEP AISWFRNGKL IEENEKYILK GSNTELTVRN IINSDGGPYV CRATNKAGED EKQAFLQVFV QPHIIQLKNE TTYENGQVTL VCDAEGEPIP EITWKRAVDG FTFTEGDKSL DGRIEVKGQH GSSSLHIKDV KLSDSGRYDC EAASRIGGHQ KSMYLDIEYA PKFISNQTIY YSWEGNPINI SCDVKSNPPA SIHWRRDKLV LPAKNTTNLK TYSTGRKMIL EIAPTSDNDF GRYNCTATNH IGTRFQEYIL ALADVPSSPY GVKIIELSQT TAKVSFNKPD SHGGVPIHHY QVDVKEVASE IWKIVRSHGV QTMVVLNNLE PNTTYEIRVA AVNGKGQGDY SKIEIFQTLP VREPSPPSIH GQPSSGKSFK LSITKQDDGG APILEYIVKY RSKDKEDQWL EKKVQGNKDH IILEHLQWTM GYEVQITAAN RLGYSEPTVY EFSMPPKPNI IKDTLFN |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCAM2重组蛋白的模拟参考文献示例(基于常见研究方向推测，非真实文献)：
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1. **标题**：*Recombinant NCAM2 Enhances Neurite Outgrowth in Hippocampal Neurons*
**作者**：Smith A, et al.
**摘要**：研究团队在大肠杆菌中成功表达并纯化重组NCAM2胞外域，通过体外神经元培养实验证明其显著促进海马神经元突触生长，提示其在神经再生中的潜在应用。
2. **标题**：*Structural Insights into NCAM2-Mediated Synaptic Adhesion*
**作者**：Zhang L, et al.
**摘要**：利用重组NCAM2蛋白进行X射线晶体学分析，解析其Ig1-2结构域的三维构象，揭示其与突触前受体相互作用的分子机制，为神经发育障碍研究提供新靶点。
3. **标题**：*NCAM2 Recombinant Protein Attenuates Amyloid-β Toxicity in Alzheimer’s Models*
**作者**：Jones R, et al.
**摘要**：在阿尔茨海默病细胞模型中，重组NCAM2通过激活Akt/mTOR通路减少β-淀粉样蛋白诱导的神经元凋亡，提示其治疗神经退行性疾病的潜力。
4. **标题**：*High-Yield Production of Functional NCAM2 in Mammalian Cells*
**作者**：Chen H, et al.
**摘要**：优化哺乳动物细胞表达系统，获得高纯度糖基化NCAM2重组蛋白，验证其与FGF受体的结合活性，为下游功能研究与药物开发提供可靠工具。
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*注：以上文献为示例性质，实际研究中需通过学术数据库检索真实文献。*
**Background of NCAM2 Recombinant Protein**
Neural Cell Adhesion Molecule 2 (NCAM2), also known as Rbms2. is a member of the immunoglobulin (Ig) superfamily of cell adhesion molecules (IgCAMs). It plays critical roles in neural development, including axon guidance, synaptic plasticity, and cell-cell interactions. Structurally, NCAM2 contains five Ig-like domains and two fibronectin type III repeats in its extracellular region, followed by a transmembrane domain and a short cytoplasmic tail. Unlike its homolog NCAM1. NCAM2 exhibits distinct expression patterns, predominantly in the olfactory system, hippocampus, and cortical regions, suggesting specialized functions in these areas.
Recombinant NCAM2 protein is engineered using molecular cloning techniques, often expressed in mammalian (e.g., HEK293) or bacterial systems to ensure proper folding and post-translational modifications. The recombinant form allows researchers to study NCAM2's interactions with ligands like heparan sulfate proteoglycans or other adhesion molecules, as well as its role in signaling pathways involving FGF receptors or cytoskeletal regulators.
Interest in NCAM2 has grown due to its implications in neurodevelopmental and neurodegenerative disorders. For instance, altered NCAM2 expression is linked to Down syndrome, autism spectrum disorders, and Alzheimer’s disease, where it may influence amyloid-beta pathology. Recombinant NCAM2 serves as a tool to investigate these mechanisms in vitro or in animal models, aiding drug discovery and diagnostic biomarker development.
In summary, NCAM2 recombinant protein provides a versatile platform to explore neural adhesion biology and disease-related pathways, bridging structural insights with therapeutic potential.
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