| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPY5R |
| Uniprot No | Q15761 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MDLELDEYYNKTLATENNTAATRNSDFPVWDDYKSSVDDLQYFLIGLYTF VSLLGFMGNLLILMALMKKRNQKTTVNFLIGNLAFSDILVVLFCSPFTLT SVLLDQWMFGKVMCHIMPFLQCVSVLVSTLILISIAIVRYHMIKHPISNN LTANHGYFLIATVWTLGFAICSPLPVFHSLVELQETFGSALLSSRYLCVE SWPSDSYRIAFTISLLLVQYILPLVCLTVSHTSVCRSISCGLSNKENRLE ENEMINLTLHPSKKSGPQVKLSGSHKWSYSFIKKHRRRYSKKTACVLPAP ERPSQENHSRILPENFGSVRSQLSSSSKFIPGVPTCFEIKPEENSDVHEL RVKRSVTRIKKRSRSVFYRLTILILVFAVSWMPLHLFHVVTDFNDNLISN RHFKLVYCICHLLGMMSCCLNPILYGFLNNGIKADLVSLIHCLHM |
| 预测分子量 | 75 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPY5R重组蛋白的3篇代表性文献摘要(模拟示例，非真实文献)：
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1. **"Expression and functional characterization of the human neuropeptide Y Y5 receptor in mammalian cells"**
*作者：Smith A, et al.*
摘要：研究成功在HEK293细胞中重组表达了人源NPY5R，验证其与神经肽Y的高亲和力结合，并证实其通过cAMP信号通路介导食欲调节功能。
2. **"Crystal structure of the NPY5R bound to a selective antagonist"**
*作者：Zhang L, et al.*
摘要：通过X射线晶体学解析了NPY5R与其小分子拮抗剂的复合物结构，揭示了受体跨膜结构域的配体结合口袋特征，为肥胖药物设计提供结构基础。
3. **"Development of a fluorescent ligand-based assay for high-throughput screening of NPY5R modulators"**
*作者：Johnson R, et al.*
摘要：利用重组NPY5R蛋白开发了一种基于荧光标记配体的高通量筛选方法，成功鉴定出多个新型拮抗剂，用于潜在抗肥胖疗法的优化。
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*注：以上文献为示例，实际引用需查询权威数据库(如PubMed)获取真实研究。*
The neuropeptide Y receptor type 5 (NPY5R) is a G protein-coupled receptor (GPCR) belonging to the neuropeptide Y (NPY) receptor family, which comprises four subtypes (Y1. Y2. Y4. Y5) in humans. NPY5R is primarily activated by neuropeptide Y (NPY) and peptide YY (PYY), two evolutionarily conserved peptides involved in regulating energy homeostasis, appetite, and metabolic processes. Structurally, it features seven transmembrane domains characteristic of GPCRs and signals through Gαi/o proteins, inhibiting adenylate cyclase activity and modulating intracellular calcium levels.
NPY5R has garnered significant attention for its role in appetite stimulation and obesity-related pathways. Studies highlight its expression in hypothalamic regions critical for feeding behavior, making it a therapeutic target for metabolic disorders. However, its physiological roles extend beyond energy balance, with emerging evidence implicating NPY5R in anxiety, stress responses, and circadian rhythm regulation.
Recombinant NPY5R proteins are engineered for in vitro studies to elucidate receptor-ligand interactions, signaling mechanisms, and drug discovery. These proteins are typically produced in mammalian or insect cell systems to ensure proper post-translational modifications and ligand-binding competence. Purified recombinant NPY5R enables high-throughput screening of agonists/antagonists, structural studies (e.g., crystallography), and functional assays assessing receptor activation or inhibition.
Despite promising preclinical data, NPY5R-targeted drugs have faced challenges in clinical trials, partly due to receptor redundancy and cross-species functional variations. Current research focuses on developing selective modulators with improved specificity and pharmacokinetic profiles. The availability of recombinant NPY5R continues to drive mechanistic insights into its dual roles in metabolism and neurobehavioral regulation, underscoring its potential in multifactorial disease interventions.
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