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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARTN |
| Uniprot No | Q5T4W7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 108-220aa |
| 氨基酸序列 | AGGPGSRARAAGARGCRLRSQLVPVRALGLGHRSDELVRFRFCSGSCRRARSPHDLSLASLLGAGALRPPPGSRPVSQPCCRPTRYEAVSFMDVNSTWRTVDRLSATACGCLG |
| 预测分子量 | 47.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARTN(Artemin)重组蛋白的3篇参考文献及其摘要概括：
1. **"Efficient production of recombinant human Artemin in Escherichia coli"**
*作者：Li Y, et al.*
摘要：该研究报道了利用大肠杆菌表达系统高效生产重组人Artemin蛋白的方法，通过优化密码子和纯化步骤，获得了高纯度蛋白，并验证其在体外促进神经元轴突生长的活性。
2. **"Artemin promotes sensory nerve regeneration via recombinant protein delivery in a rodent model"**
*作者：Wang H, et al.*
摘要：研究通过重组ARTN蛋白局部递送，证明其在周围神经损伤模型中显著增强感觉神经再生和功能恢复，提示其在神经修复治疗中的潜在应用价值。
3. **"Structural basis of Artemin-GFRα3 interactions and implications for pain signaling"**
*作者：Smith J, et al.*
摘要：通过晶体学解析ARTN与其受体GFRα3的复合物结构，揭示了关键结合域，并证明重组ARTN蛋白通过此通路调控慢性疼痛信号，为靶向药物设计提供依据。
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注：上述文献为示例，实际引用需根据具体研究匹配真实发表的论文。
ARTN (Artemin) is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family, which includes GDNF, neurturin, and persephin. It plays critical roles in nervous system development, maintenance, and repair by activating the RET tyrosine kinase receptor through a multi-component complex involving GDNF family receptor alpha-3 (GFRα3). ARTN is particularly associated with promoting neuronal survival, axon guidance, and nociceptive sensory neuron development. Its expression is observed in peripheral tissues and the central nervous system, with dysregulation linked to neuropathic pain, neurodegenerative disorders, and cancer progression.
Recombinant ARTN protein is typically produced using engineered expression systems (e.g., E. coli, mammalian cells) to ensure proper folding and bioactivity. The purified protein retains the ability to bind GFRα3 and activate downstream signaling pathways like MAPK/ERK and PI3K/AKT. Researchers utilize ARTN recombinant proteins to study neuroregeneration mechanisms, pain sensitivity pathways, and cancer biology, particularly in tumors with RET or GFRα3 overexpression, such as breast cancer and neuroblastoma. Recent studies also explore its therapeutic potential in nerve injury models and chemotherapy-induced neuropathy. Structural analyses often highlight conserved cysteine residues critical for its tertiary structure and receptor interaction. Commercial preparations usually undergo rigorous endotoxin testing and functional validation (e.g., neurite outgrowth assays) to ensure experimental reproducibility.
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