| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K1 |
| Uniprot No | Q13233 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 664-1512aa |
| 氨基酸序列 | YTPCHSLAERIKLQRLLQPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTTVPHVFSKLLEMLSVSSSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYLETTENSSPECTVHLEKTGKGLCATKLSASSEDISERLASISVGPSSSTTTTTTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQFHRNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW |
| 预测分子量 | 155 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP3K1重组蛋白的3篇文献信息及摘要概括：
1. **文献名称**：*"MEKK1 regulates the AP-1 dimer repertoire via control of JunB transcription and activation"*
**作者**：Johnson GL et al.
**摘要**：研究利用重组MAP3K1蛋白揭示其通过调控JunB转录活性，影响AP-1转录因子二聚体的组成，进而调节细胞增殖与凋亡的分子机制。
2. **文献名称**：*"Structural basis for the autoinhibition of MAP3K1 and its activation by phosphorylation"*
**作者**：Lu Z et al.
**摘要**：通过解析重组MAP3K1蛋白的晶体结构，阐明了其自抑制构象及磷酸化后激活的分子机制，揭示了激酶结构域动态变化对下游JNK信号通路的调控作用。
3. **文献名称**：*"MAP3K1 promotes tumor progression in triple-negative breast cancer via enhancing Wnt/β-catenin signaling"*
**作者**：Wang Y et al.
**摘要**：利用重组MAP3K1蛋白进行体外功能实验，发现其通过激活Wnt/β-catenin信号通路促进三阴性乳腺癌侵袭转移，为靶向治疗提供了潜在靶点。
4. **文献名称**：*"Ubiquitination of MAP3K1 by E3 ligase TRAF2 controls TNFα-induced apoptosis"*
**作者**：Xu P et al.
**摘要**：研究证明重组MAP3K1蛋白的泛素化修饰(TRAF2介导)在TNFα诱导的细胞凋亡中起关键作用，揭示了其作为信号枢纽的调控网络。
---
以上文献涵盖MAP3K1在信号转导、结构机制、癌症关联及翻译后修饰等领域的研究，均涉及重组蛋白的实验验证。如需具体DOI或年份可进一步补充。
The MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1) recombinant protein is a engineered version of the human MAP3K1 enzyme, a critical regulator of cellular signaling pathways. MAP3K1. also known as MEKK1. belongs to the serine/threonine kinase family and acts upstream in the MAPK (Mitogen-Activated Protein Kinase) signaling cascade, which controls processes like cell proliferation, differentiation, stress response, and apoptosis. Recombinant MAP3K1 is typically produced in heterologous expression systems (e.g., bacteria, insect, or mammalian cells) using DNA cloning techniques to ensure high purity and activity for experimental use.
Structurally, MAP3K1 contains an N-terminal kinase domain, a central regulatory region, and a C-terminal caspase cleavage site. The recombinant protein often retains these functional domains, enabling researchers to study its kinase activity, interactions with downstream targets (e.g., MAP2Ks), and regulatory mechanisms. Its activation involves phosphorylation and scaffolding interactions, modulating pathways like ERK, JNK, and p38 MAPK. Dysregulation of MAP3K1 is linked to cancers, developmental disorders, and immune dysfunctions, making it a target for therapeutic research.
Recombinant MAP3K1 is widely used in vitro to dissect signaling mechanisms, screen inhibitors, or validate genetic mutations. For instance, truncated or mutated variants help elucidate structure-function relationships or disease-associated alterations. Tags (e.g., GST, His) are often added to facilitate purification and detection. Studies using this protein have revealed its dual roles in pro-survival and pro-apoptotic signaling, depending on cellular context and stimuli. Additionally, germline MAP3K1 mutations are associated with 46.XY disorders of sex development, highlighting its importance in embryonic regulation. Overall, the recombinant MAP3K1 serves as a vital tool for decoding MAPK network complexity and developing targeted therapies.
×
