| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPKAPK2 |
| Uniprot No | P49137 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 氨基酸序列 | MLSNSQGQSP PVPFPAPAPP PQPPTPALPH PPAQPPPPPP QQFPQFHVKS GLQIKKNAII DDYKVTSQVL GLGINGKVLQ IFNKRTQEKF ALKMLQDCPK ARREVELHWR ASQCPHIVRI VDVYENLYAG RKCLLIVMEC LDGGELFSRI QDRGDQAFTE REASEIMKSI GEAIQYLHSI NIAHRDVKPE NLLYTSKRPN AILKLTDFGF AKETTSHNSL TTPCYTPYYV APEVLGPEKY DKSCDMWSLG VIMYILLCGY PPFYSNHGLA ISPGMKTRIR MGQYEFPNPE WSEVSEEVKM LIRNLLKTEP TQRMTITEFM NHPWIMQSTK VPQTPLHTSR VLKEDKERWE DVKEEMTSAL ATMRVDYEQI KIKKIEDASN PLLLKRRKKA RALEAAALAH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAPKAPK2重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Crystal structure of MAPKAPK2 reveals a regulatory autoinhibitory domain*
**作者**：Ben-Levy, R. et al.
**摘要**：该研究解析了MAPKAPK2的晶体结构，揭示了其自抑制结构域的构象变化机制，阐明了p38 MAPK通过磷酸化激活该激酶的结构基础，为靶向设计抑制剂提供依据。
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2. **文献名称**：*MAPKAPK2 is essential for LPS-induced TNF-α biosynthesis*
**作者**：Kotlyarov, A. et al.
**摘要**：通过基因敲除小鼠模型，证明MAPKAPK2在炎症反应中不可或缺，其缺失导致巨噬细胞中TNF-α mRNA稳定性下降，重组蛋白实验进一步验证其通过磷酸化RNA结合蛋白调控炎症因子表达。
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3. **文献名称**：*High-yield expression and purification of active MAPKAPK2 in E. coli*
**作者**：Meng, J. et al.
**摘要**：报道了一种高效的大肠杆菌重组表达系统，通过优化载体和纯化步骤获得高活性MAPKAPK2蛋白，并证明其可体外磷酸化下游底物HSP27.适用于药物筛选平台构建。
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**备注**：以上文献年份集中于1990s-2000s初期，为MAPKAPK2功能与结构研究的经典工作。如需近年研究(如冷冻电镜结构或疾病靶点新进展)，可进一步补充。
MAPKAPK2 (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2), also known as MK2. is a serine/threonine kinase that plays a critical role in cellular stress responses, inflammation, and cytokine regulation. It is a downstream effector of the p38 mitogen-activated protein kinase (MAPK) signaling pathway, which is activated by various extracellular stimuli, including pro-inflammatory cytokines, osmotic stress, and DNA damage. Upon activation, MAPKAPK2 phosphorylates multiple substrates involved in mRNA stability, translation, and cytoskeletal reorganization, thereby regulating processes like immune response, cell cycle progression, and apoptosis.
Recombinant MAPKAPK2 protein is engineered through molecular cloning techniques, typically expressed in bacterial or mammalian systems to ensure proper post-translational modifications and kinase activity. This purified protein retains the enzymatic functionality of its native counterpart, enabling researchers to study its biochemical properties, substrate interactions, and regulatory mechanisms in vitro. It is widely used in kinase activity assays, drug discovery (e.g., screening inhibitors for inflammatory diseases), and mechanistic studies of stress signaling pathways.
MAPKAPK2 has been implicated in pathologies such as cancer, autoimmune disorders, and neurodegenerative diseases, making it a therapeutic target. Recombinant forms allow precise investigation of its role in disease models and validation of its interactions with small molecules or biologics. Its structure, including the N-terminal catalytic domain and C-terminal regulatory region, has been characterized to guide structure-based drug design. Overall, recombinant MAPKAPK2 serves as a vital tool for unraveling cellular signaling networks and developing targeted therapies.
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