| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK10 |
| Uniprot No | P53779 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-464aa |
| 氨基酸序列 | MSLHFLYYCS EPTLDVKIAF CQGFDKQVDV SYIAKHYNMS KSKVDNQFYS VEVGDSTFTV LKRYQNLKPI GSGAQGIVCA AYDAVLDRNV AIKKLSRPFQ NQTHAKRAYR ELVLMKCVNH KNIISLLNVF TPQKTLEEFQ DVYLVMELMD ANLCQVIQME LDHERMSYLL YQMLCGIKHL HSAGIIHRDL KPSNIVVKSD CTLKILDFGL ARTAGTSFMM TPYVVTRYYR APEVILGMGY KENVDIWSVG CIMGEMVRHK ILFPGRDYID QWNKVIEQLG TPCPEFMKKL QPTVRNYVEN RPKYAGLTFP KLFPDSLFPA DSEHNKLKAS QARDLLSKML VIDPAKRISV DDALQHPYIN VWYDPAEVEA PPPQIYDKQL DEREHTIEEW KELIYKEVMN SEEKTKNGVV KGQPSPSGAA VNSSESLPPS SSVNDISSMS TDQTLASDTD SSLEASAGPL GCCR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAPK10重组蛋白的3篇参考文献示例(内容为模拟生成，仅供参考)：
1. **文献名称**：*Expression and Purification of Active Human MAPK10/JNK3 in Escherichia coli*
**作者**：Chen L, Wang H, et al.
**摘要**：本研究报道了利用大肠杆菌表达系统高效表达并纯化具有激酶活性的人源MAPK10重组蛋白。通过优化诱导条件和亲和层析技术，获得高纯度蛋白，并验证其磷酸化c-Jun的能力，为体外激酶实验提供可靠工具。
2. **文献名称**：*Structural Insights into MAPK10 Activation by Co-crystallization with ATP-analog*
**作者**：Smith K, Tanaka F, et al.
**摘要**：作者解析了MAPK10重组蛋白与ATP类似物的复合物晶体结构，揭示了其激酶结构域的构象变化及活性位点特征，为开发靶向JNK3的小分子抑制剂提供了结构基础。
3. **文献名称**：*MAPK10 Recombinant Protein Attenuates Neuronal Apoptosis in Parkinson’s Disease Models*
**作者**：Zhang R, Liu Y, et al.
**摘要**：通过体外神经元模型证明，外源性添加MAPK10重组蛋白可调控Bcl-2/Bax通路，减少多巴胺能神经元凋亡，提示其在神经退行性疾病中的潜在治疗价值。
(注：以上文献信息为示例性内容，实际引用时请核实真实文献。)
**Background of MAPK10 Recombinant Protein**
MAPK10 (mitogen-activated protein kinase 10), also known as c-Jun N-terminal kinase 3 (JNK3), is a member of the serine/threonine protein kinase family. It plays a critical role in stress-response signaling pathways, regulating cellular processes such as apoptosis, differentiation, and inflammation. Unlike JNK1 and JNK2. which are ubiquitously expressed, JNK3 is predominantly localized in the brain, heart, and testis, making it a key player in neuronal function and neurodegenerative diseases like Alzheimer’s and Parkinson’s.
Recombinant MAPK10 protein is engineered through genetic cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form. This allows researchers to study its enzymatic activity, substrate interactions, and regulatory mechanisms *in vitro*. The recombinant protein retains functional domains, including the kinase domain responsible for phosphorylating downstream targets like transcription factors (e.g., c-Jun) or other kinases.
Its applications span drug discovery, particularly in identifying inhibitors for neurodegenerative or inflammatory disorders, and mechanistic studies of JNK-dependent signaling cascades. Researchers also use it to investigate stress-induced apoptosis or synaptic plasticity in neurological models. Quality-controlled batches ensure consistency in experiments, overcoming variability associated with native protein isolation. Tags (e.g., GST, His-tag) are often added for ease of purification and detection.
Overall, MAPK10 recombinant protein serves as a vital tool for dissecting JNK3’s role in health and disease, bridging molecular insights with therapeutic development.
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