| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPTLC3 |
| Uniprot No | Q9NUV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-552aa |
| 氨基酸序列 | MANPGGGAVCNGKLHNHKKQSNGSQSRNCTKNGIVKEAQQNGKPHFYDKLIVESFEEAPLHVMVFTYMGYGIGTLFGYLRDFLRNWGIEKCNAAVERKEQKDFVPLYQDFENFYTRNLYMRIRDNWNRPICSAPGPLFDLMERVSDDYNWTFRFTGRVIKDVINMGSYNFLGLAAKYDESMRTIKDVLEVYGTGVASTRHEMGTLDKHKELEDLVAKFLNVEAAMVFGMGFATNSMNIPALVGKGCLILSDELNHTSLVLGARLSGATIRIFKHNNTQSLEKLLRDAVIYGQPRTRRAWKKILILVEGVYSMEGSIVHLPQIIALKKKYKAYLYIDEAHSIGAVGPTGRGVTEFFGLDPHEVDVLMGTFTKSFGASGGYIAGRKDLVDYLRVHSHSAVYASSMSPPIAEQIIRSLKLIMGLDGTTQGLQRVQQLAKNTRYFRQRLQEMGFIIYGNENASVVPLLLYMPGKVAAFARHMLEKKIGVVVVGFPATPLAEARARFCVSAAHTREMLDTVLEALDEMGDLLQLKYSRHKKSARPELYDETSFELED |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPTLC3重组蛋白的3篇示例参考文献(注：内容为示例性虚构，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Heterologous Expression and Functional Characterization of Human SPTLC3 in Yeast"*
**作者**: Chen, L., et al.
**摘要**: 本研究在酵母系统中成功表达并纯化了人源SPTLC3重组蛋白，证实其与SPTLC1亚基形成复合物后可恢复鞘脂合成活性，揭示了SPTLC3在鞘氨醇代谢中的关键作用及底物特异性。
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2. **文献名称**: *"Structural Insights into SPTLC3 through Cryo-EM Analysis of Recombinant Protein Complexes"*
**作者**: Wang, Y., & Patel, S.
**摘要**: 通过冷冻电镜技术解析了SPTLC3-SPTLC1重组蛋白复合体的三维结构，发现SPTLC3的跨膜结构域可能参与底物通道的形成，为开发靶向鞘脂合成的抑制剂提供了结构基础。
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3. **文献名称**: *"SPTLC3 Recombinant Protein Overexpression Promotes Ceramide Accumulation in Hepatocellular Carcinoma Cells"*
**作者**: Kim, H., et al.
**摘要**: 利用大肠杆菌表达系统制备功能性SPTLC3重组蛋白，并在肝癌细胞中验证其过表达导致神经酰胺水平升高，提示SPTLC3可能通过调控鞘脂代谢影响肿瘤细胞凋亡通路。
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建议通过PubMed或Web of Science搜索关键词“SPTLC3 recombinant”或“SPTLC3 expression”获取真实文献。如需进一步协助筛选，请提供具体研究方向。
**Background of SPTLC3 Recombinant Protein**
Serine palmitoyltransferase long-chain subunit 3 (SPTLC3) is a key enzyme component in the biosynthesis of sphingolipids, a class of lipids critical for membrane structure, cell signaling, and apoptosis. SPTLC3 is one of three homologous subunits (SPTLC1. SPTLC2. SPTLC3) that form the serine palmitoyltransferase (SPT) complex, which catalyzes the first and rate-limiting step in sphingolipid synthesis: the condensation of serine and palmitoyl-CoA to produce 3-ketodihydrosphingosine. Unlike SPTLC1 and SPTLC2. which are ubiquitously expressed, SPTLC3 exhibits tissue-specific expression, with higher levels observed in the testes, placenta, and certain cancer cells. This subunit is thought to modulate SPT activity by altering substrate specificity, enabling the incorporation of alternative fatty acyl-CoA substrates (e.g., myristoyl-CoA or C16:1-CoA) into sphingoid bases, thereby diversifying sphingolipid profiles.
Recombinant SPTLC3 protein is engineered for in vitro studies to dissect its structural, enzymatic, and regulatory roles. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the purified protein retains catalytic activity when reconstituted with other SPT subunits. Researchers utilize SPTLC3 recombinant protein to investigate its unique biochemical properties, substrate preferences, and interactions with regulatory proteins like ORMDL3. Its role in diseases, particularly cancers linked to dysregulated sphingolipid metabolism (e.g., colon or liver cancers), has spurred interest in targeting SPTLC3 for therapeutic intervention. Additionally, studies explore its involvement in rare genetic disorders, such as hereditary sensory and autonomic neuropathies (HSAN), where SPT mutations disrupt sphingolipid homeostasis. By enabling precise mechanistic studies, SPTLC3 recombinant protein serves as a vital tool for advancing lipid biology and translational research.
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